Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   HeN100_RS23635 Genome accession   NZ_CP044443
Coordinates   4494200..4496029 (-) Length   609 a.a.
NCBI ID   WP_077250084.1    Uniprot ID   -
Organism   Escherichia coli strain HeN100     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4489200..4501029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HeN100_RS23610 (HeN100_23865) bioP 4489918..4490817 (-) 900 WP_001196238.1 biotin transporter -
  HeN100_RS23615 (HeN100_23870) yigL 4490893..4491693 (-) 801 WP_000285362.1 sugar/pyridoxal phosphate phosphatase YigL -
  HeN100_RS23620 (HeN100_23875) pldB 4491701..4492723 (-) 1023 WP_000487654.1 lysophospholipase L2 -
  HeN100_RS23625 (HeN100_23880) rhtB 4492834..4493454 (+) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  HeN100_RS23630 (HeN100_23885) rhtC 4493516..4494136 (-) 621 WP_000928824.1 threonine export protein RhtC -
  HeN100_RS23635 (HeN100_23890) recQ 4494200..4496029 (-) 1830 WP_077250084.1 ATP-dependent DNA helicase RecQ Machinery gene
  HeN100_RS23640 (HeN100_23895) pldA 4496162..4497031 (-) 870 WP_001259700.1 phospholipase A -
  HeN100_RS23645 (HeN100_23900) yigI 4497196..4497663 (+) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  HeN100_RS23650 (HeN100_23905) rarD 4497715..4498605 (+) 891 WP_112922312.1 EamA family transporter RarD -
  HeN100_RS23655 (HeN100_23910) yigG 4498700..4499080 (+) 381 WP_000944218.1 protein YigG -
  HeN100_RS23660 (HeN100_23915) yigF 4499094..4499474 (+) 381 WP_000032581.1 DUF2628 domain-containing protein -
  HeN100_RS23665 (HeN100_23920) corA 4499517..4500467 (-) 951 WP_000947159.1 magnesium/cobalt transporter CorA -
  HeN100_RS23670 ysgD 4500619..4500675 (-) 57 WP_211180520.1 protein YsgD -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68333.48 Da        Isoelectric Point: 7.2544

>NTDB_id=342586 HeN100_RS23635 WP_077250084.1 4494200..4496029(-) (recQ) [Escherichia coli strain HeN100]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
AALIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=342586 HeN100_RS23635 WP_077250084.1 4494200..4496029(-) (recQ) [Escherichia coli strain HeN100]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACCGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACAGCAACTTGAAGT
GATGACAGGCTGCCGCACCGGGCAAATTCGCTTACTGTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTCGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAGTTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAGCGCGGTAAGTCAGGCATT
ATCTACTGCAACAGCCGCGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTTCGCGCCGATGTGCAGGAAAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAGCCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

RQC domain (RQC)

(406-514)

HRDC domain (HRDC)

(533-599)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.034

97.209

0.389