Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HeN100_RS20100 Genome accession   NZ_CP044443
Coordinates   3754277..3754717 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain HeN100     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3749277..3759717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HeN100_RS20080 (HeN100_20345) aroP 3750275..3751648 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  HeN100_RS20085 (HeN100_20350) ampE 3751691..3752545 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  HeN100_RS20090 (HeN100_20355) ampD 3752542..3753093 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HeN100_RS20095 (HeN100_20360) nadC 3753181..3754074 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HeN100_RS20100 (HeN100_20365) pilA 3754277..3754717 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  HeN100_RS20105 (HeN100_20370) pilB 3754727..3756112 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  HeN100_RS20110 (HeN100_20375) hofC 3756102..3757304 (+) 1203 WP_000157266.1 protein transport protein HofC -
  HeN100_RS20115 (HeN100_20380) guaC 3757339..3758382 (-) 1044 WP_001217338.1 GMP reductase -
  HeN100_RS20120 - 3758538..3758582 (-) 45 WP_120795372.1 protein YacM -
  HeN100_RS20125 (HeN100_20385) coaE 3758607..3759227 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=342571 HeN100_RS20100 WP_000360904.1 3754277..3754717(+) (pilA) [Escherichia coli strain HeN100]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=342571 HeN100_RS20100 WP_000360904.1 3754277..3754717(+) (pilA) [Escherichia coli strain HeN100]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363