Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FNW43_RS03840 Genome accession   NZ_CP044410
Coordinates   824475..824915 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain ecMN1F     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 819475..829915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FNW43_RS03820 (FNW43_03820) coaE 819965..820585 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  FNW43_RS22645 - 820610..820654 (+) 45 WP_120795372.1 protein YacM -
  FNW43_RS03825 (FNW43_03825) guaC 820810..821853 (+) 1044 WP_001217338.1 GMP reductase -
  FNW43_RS03830 (FNW43_03830) hofC 821888..823090 (-) 1203 WP_000157266.1 protein transport protein HofC -
  FNW43_RS03835 (FNW43_03835) pilB 823080..824465 (-) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  FNW43_RS03840 (FNW43_03840) pilA 824475..824915 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  FNW43_RS03845 (FNW43_03845) nadC 825118..826011 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FNW43_RS03850 (FNW43_03850) ampD 826099..826650 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FNW43_RS03855 (FNW43_03855) ampE 826647..827501 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  FNW43_RS03860 (FNW43_03860) aroP 827544..828917 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=342452 FNW43_RS03840 WP_000360904.1 824475..824915(-) (pilA) [Escherichia coli strain ecMN1F]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=342452 FNW43_RS03840 WP_000360904.1 824475..824915(-) (pilA) [Escherichia coli strain ecMN1F]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment