Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BACIH_RS08480 Genome accession   NZ_CP044360
Coordinates   1719825..1720259 (+) Length   144 a.a.
NCBI ID   WP_012117601.1    Uniprot ID   A7Z4Z1
Organism   Bacillus amyloliquefaciens strain V167     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1714825..1725259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BACIH_RS08475 (BACIH_1724) - 1718325..1719536 (-) 1212 WP_012117600.1 cytochrome P450 -
  BACIH_RS08480 (BACIH_1726) nucA/comI 1719825..1720259 (+) 435 WP_012117601.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BACIH_RS08485 (BACIH_1727) - 1720319..1721074 (+) 756 WP_023356903.1 YoaK family protein -
  BACIH_RS08490 (BACIH_1728) - 1721108..1721470 (-) 363 WP_007410383.1 hypothetical protein -
  BACIH_RS08495 (BACIH_1730) - 1721663..1722991 (-) 1329 WP_012117603.1 S8 family peptidase -
  BACIH_RS08500 (BACIH_1731) - 1723170..1723403 (+) 234 WP_015239897.1 hypothetical protein -
  BACIH_RS08505 (BACIH_1733) - 1723658..1724365 (+) 708 WP_012117604.1 poly-gamma-glutamate hydrolase family protein -
  BACIH_RS08510 (BACIH_1734) - 1724425..1724877 (+) 453 WP_012117605.1 OsmC family protein -
  BACIH_RS08515 (BACIH_1735) - 1724883..1725245 (-) 363 WP_150978880.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15562.48 Da        Isoelectric Point: 7.2418

>NTDB_id=342271 BACIH_RS08480 WP_012117601.1 1719825..1720259(+) (nucA/comI) [Bacillus amyloliquefaciens strain V167]
MNAFMKWAASLLLVISLQFGFTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=342271 BACIH_RS08480 WP_012117601.1 1719825..1720259(+) (nucA/comI) [Bacillus amyloliquefaciens strain V167]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCTTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAAAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z4Z1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

58.974

81.25

0.479