Detailed information NTG0342257

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: BACIH_RS07720
• Genome accession: NZ_CP044360
• Coordinates: 1505911..1508322 (+)
• Length: 803 a.a.
• NCBI ID: WP_012117529.1
• Uniprot ID: A7Z4J1
• Organism: Bacillus amyloliquefaciens strain V167
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1500911..1513322

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BACIH_RS07695 (BACIH_1565)	-	1502553..1503428 (+)	876	WP_007409745.1	YicC/YloC family endoribonuclease	-
BACIH_RS07700 (BACIH_1566)	remA	1503505..1503774 (+)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
BACIH_RS07705 (BACIH_1567)	gmk	1503782..1504396 (+)	615	WP_003154347.1	guanylate kinase	-
BACIH_RS07710 (BACIH_1568)	rpoZ	1504400..1504603 (+)	204	WP_003154346.1	DNA-directed RNA polymerase subunit omega	-
BACIH_RS07715 (BACIH_1569)	coaBC	1504694..1505914 (+)	1221	WP_012117528.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
BACIH_RS07720 (BACIH_1570)	priA	1505911..1508322 (+)	2412	WP_012117529.1	primosomal protein N'	Machinery gene
BACIH_RS07725 (BACIH_1571)	def	1508347..1508829 (+)	483	WP_012117530.1	peptide deformylase	-
BACIH_RS07730 (BACIH_1573)	fmt	1508834..1509786 (+)	953	Protein_1484	methionyl-tRNA formyltransferase	-
BACIH_RS07735 (BACIH_1574)	rsmB	1509773..1511116 (+)	1344	WP_012117532.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
BACIH_RS07740 (BACIH_1575)	rlmN	1511120..1512211 (+)	1092	WP_003154339.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
BACIH_RS07745 (BACIH_1576)	-	1512217..1512978 (+)	762	WP_041482191.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-

Sequence

Protein

• Length: 803 a.a.
• Molecular weight: 90819.85 Da
• Isoelectric Point: 8.8072

>NTDB_id=342257 BACIH_RS07720 WP_012117529.1 1505911..1508322(+) (priA) [Bacillus amyloliquefaciens strain V167]
MNVAEVIVDVSSKNIDRPFDYKIPDHLKGMIEVGMRVIVPFGPRKLQGFVTGLKESSELNGGSMKEVEKLLDLTPVLTEE
LMQLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKVVDEEALTPDIKQLFRNQKSLLYSDITDESILSSLQKFVRKGS
IDVTYKVAQKTNKKMIRMIEAGADKEELARHADALTKQASKQLTVLTYFIGGGAKISAAELCKKTDSSSATLKTLLKKGL
LTESFEEVYRDPYQDRMFKKTEPLSLTEEQQAAFEPIKQAVADHVHHVFLLHGVTGSGKTEIYLQSIEKVLAKGQEAIVL
VPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIQRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQEE
MPRYHAKDVAIRRAEHHSCPVVLGSATPTLESFARAKKGVYELLPLKHRVNRQLMPDVSLVDMREELRNGNRSMFSRELM
EQLEEKLAKKEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPGTCPECGSEHIRFFG
TGTQRVEEELTKVLPQARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIPDF
RAAEKTFQLLTQVSGRAGRHEKPGTVVIQTYTPSHYSIQLTKTHDYETFFQQEMAHRRAQSYPPYYYVALVTVSHEEAAK
AAVTAEKITNYLRAGCGPDTKILGPAASPIARIKDRYRYQCVIKYRQENELQIQLKKILEHYKRDIAQKHVMISIDMNPY
MLM

Nucleotide

• Length: 2412 bp

>NTDB_id=342257 BACIH_RS07720 WP_012117529.1 1505911..1508322(+) (priA) [Bacillus amyloliquefaciens strain V167]
ATGAATGTAGCAGAAGTCATTGTCGATGTCAGCTCCAAAAACATTGACAGACCCTTTGATTACAAAATTCCCGATCATTT
AAAAGGGATGATCGAAGTCGGCATGCGGGTGATTGTCCCGTTTGGCCCGCGCAAGCTTCAAGGATTTGTGACCGGTTTGA
AAGAATCGTCTGAATTAAACGGCGGCTCAATGAAGGAAGTCGAGAAGCTTCTTGATTTGACGCCCGTTCTGACTGAGGAA
CTGATGCAGCTGTCTTCATGGCTTTCTGACAAAACGCTGTCGTTTAAAATCACGGCGCTTCAGGCAATGCTGCCCGCCGC
ACTGAAGGCGAAGTACGAGAAAGAGCTGAAAGTCGTTGACGAAGAAGCGCTGACACCGGACATCAAACAGCTTTTCAGGA
ATCAAAAAAGTCTTCTGTACTCGGATATTACCGATGAAAGCATTCTTTCGTCACTTCAAAAATTCGTGCGCAAAGGAAGC
ATTGATGTCACATATAAAGTCGCCCAAAAAACGAATAAAAAAATGATCCGCATGATCGAAGCGGGGGCGGACAAAGAAGA
ACTCGCCCGGCACGCAGATGCATTGACAAAGCAGGCTTCTAAACAGCTCACCGTGCTTACTTATTTTATCGGCGGGGGAG
CAAAGATTTCCGCGGCCGAGCTTTGCAAAAAGACCGATTCCAGTTCCGCGACGCTGAAAACGCTTCTTAAAAAGGGGCTT
CTTACGGAAAGCTTTGAGGAGGTTTACCGCGATCCTTATCAGGACAGAATGTTCAAAAAAACAGAGCCGCTCTCTCTTAC
AGAAGAGCAGCAGGCCGCTTTTGAGCCGATTAAACAAGCTGTAGCCGATCATGTCCATCACGTCTTTCTGCTTCACGGCG
TCACGGGAAGCGGCAAAACGGAAATTTACCTGCAATCCATTGAAAAAGTGCTGGCAAAAGGACAAGAAGCCATCGTGCTC
GTTCCGGAAATCTCGTTGACACCGCAAATGGTCAACCGTTTTAAAGGCCGCTTCGGCTCACAGGTTGCGGTCATGCACAG
CGGATTATCCACAGGTGAAAAATACGATGAATGGCGGAAGATTCAGCGCAAGGAAGTCCGGCTTGTCGTCGGAGCCAGAT
CGGCCATCTTTGCCCCGTTTGAAAATCTCGGCATGATCATTATTGATGAAGAGCACGAATCTTCCTATAAGCAGGAGGAA
ATGCCGCGGTATCATGCAAAGGACGTAGCCATCCGCCGGGCCGAGCATCACAGCTGTCCGGTCGTCCTCGGGAGCGCCAC
GCCGACATTGGAGTCATTTGCGCGGGCGAAAAAAGGCGTTTATGAGCTGCTTCCATTAAAACATCGCGTAAATCGCCAGC
TCATGCCTGACGTTTCTCTCGTTGATATGAGAGAAGAACTGAGAAACGGCAACAGATCAATGTTTTCAAGAGAACTGATG
GAACAGCTGGAAGAGAAGCTTGCAAAAAAAGAGCAGGCCGTCCTTTTTCTGAATAAACGGGGGTATTCCTCGTTTGTGAT
GTGCCGGGACTGCGGTTATGTGCCGCAATGTCCGCATTGCGACATTTCGATGACTTATCACCGCTACGGACAAAGGCTGA
AATGCCATTACTGCGGCCATGAGGAACCTGTCCCGGGAACATGCCCGGAATGCGGCAGCGAACATATCCGCTTTTTCGGC
ACGGGAACGCAGCGTGTGGAGGAGGAGCTTACCAAAGTGCTGCCTCAGGCGCGCGTGATCAGGATGGATGTGGACACCAC
TTCAAGAAAAGGCGCCCATGAAAAACTGTTGTCAGCGTTCGGTGAAGGGAAGGCGGATATCCTGCTCGGTACGCAAATGA
TCGCAAAAGGGCTTGATTTTCCGAACGTGACGCTCGTCGGAGTATTAAGCGCAGATACGACGCTTCACATCCCTGATTTC
AGGGCCGCGGAGAAGACATTCCAGCTGCTGACGCAGGTAAGCGGCCGGGCCGGGCGGCATGAAAAGCCGGGAACGGTGGT
CATTCAAACGTATACACCGTCTCATTACAGCATTCAGCTGACGAAAACGCACGACTATGAAACTTTTTTTCAGCAGGAAA
TGGCGCACAGAAGGGCCCAGTCATACCCGCCTTATTATTACGTGGCGCTGGTGACCGTTTCTCATGAAGAAGCGGCAAAA
GCCGCGGTCACCGCAGAAAAGATTACAAACTATTTAAGGGCGGGATGCGGGCCTGATACGAAAATTCTCGGGCCGGCGGC
TTCCCCGATCGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAGACAGGAAAACGAGTTGCAGATTC
AGTTGAAAAAGATACTGGAGCACTATAAACGCGACATTGCGCAAAAGCATGTCATGATTTCAATCGATATGAATCCGTAC
ATGCTGATGTAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (562-660)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (517-543)

• Primosomal protein N C-terminal domain (PriA_C) (704-799)

• Type III restriction enzyme, res subunit (ResIII) (265-429)

3D structure

• AlphaFold DB: A7Z4J1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	84.348	100	0.846