Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   F7D03_RS05645 Genome accession   NZ_CP044314
Coordinates   1149797..1150456 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain RM9245     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1144797..1155456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F7D03_RS05625 (F7D03_05625) plsC 1144944..1145681 (-) 738 WP_000965717.1 1-acylglycerol-3-phosphate O-acyltransferase -
  F7D03_RS05630 (F7D03_05630) parC 1145914..1148172 (-) 2259 WP_096260298.1 DNA topoisomerase IV subunit A -
  F7D03_RS05635 (F7D03_05635) ygiV 1148718..1149200 (-) 483 WP_000183499.1 GyrI-like domain-containing protein -
  F7D03_RS05640 (F7D03_05640) ygiW 1149253..1149645 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  F7D03_RS05645 (F7D03_05645) ciaR 1149797..1150456 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  F7D03_RS05650 (F7D03_05650) qseC 1150453..1151802 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  F7D03_RS05655 (F7D03_05655) ygiZ 1151848..1152180 (-) 333 WP_000912123.1 DUF2645 family protein -
  F7D03_RS05660 (F7D03_05660) mdaB 1152499..1153080 (+) 582 WP_000065440.1 NADPH:quinone oxidoreductase MdaB -
  F7D03_RS05665 (F7D03_05665) ygiN 1153111..1153425 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  F7D03_RS05670 (F7D03_05670) - 1153469..1154800 (-) 1332 WP_000778880.1 MFS transporter -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=342005 F7D03_RS05645 WP_001221502.1 1149797..1150456(+) (ciaR) [Escherichia coli strain RM9245]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=342005 F7D03_RS05645 WP_001221502.1 1149797..1150456(+) (ciaR) [Escherichia coli strain RM9245]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment