Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ETT59_RS01195 Genome accession   NZ_CP035440
Coordinates   209530..210891 (+) Length   453 a.a.
NCBI ID   WP_136276563.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain emm124     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 177006..209449 209530..210891 flank 81


Gene organization within MGE regions


Location: 177006..210891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETT59_RS01035 (ETT59_01035) polA 177006..179648 (+) 2643 WP_136276507.1 DNA polymerase I -
  ETT59_RS01040 (ETT59_01040) - 179835..180290 (+) 456 WP_136268439.1 CoA-binding protein -
  ETT59_RS01045 (ETT59_01045) perR 180342..180809 (+) 468 WP_002986328.1 peroxide-responsive transcriptional repressor PerR -
  ETT59_RS01050 (ETT59_01050) - 181028..181264 (+) 237 WP_014635252.1 hypothetical protein -
  ETT59_RS01055 (ETT59_01055) - 181485..182819 (+) 1335 WP_136276506.1 phosphoadenosine phosphosulfate reductase -
  ETT59_RS01060 (ETT59_01060) - 182812..183342 (+) 531 WP_002987925.1 ParB/RepB/Spo0J family partition protein -
  ETT59_RS08980 - 183389..183538 (-) 150 Protein_157 ISL3 family transposase -
  ETT59_RS01070 (ETT59_01070) - 183670..184821 (-) 1152 Protein_158 IS3 family transposase -
  ETT59_RS01080 (ETT59_01080) - 184899..186209 (-) 1311 WP_002984130.1 SLC13 family permease -
  ETT59_RS01085 (ETT59_01085) nadC 186433..187305 (+) 873 WP_085614024.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ETT59_RS01090 (ETT59_01090) - 187605..188946 (-) 1342 Protein_161 IS3 family transposase -
  ETT59_RS01095 (ETT59_01095) - 189031..189894 (-) 864 WP_031488685.1 DUF975 family protein -
  ETT59_RS09240 (ETT59_01100) - 189931..190116 (+) 186 WP_002986321.1 hypothetical protein -
  ETT59_RS01105 (ETT59_01105) tgt 190113..191255 (+) 1143 WP_011528208.1 tRNA guanosine(34) transglycosylase Tgt -
  ETT59_RS01110 (ETT59_01110) - 191472..191783 (+) 312 WP_002987947.1 CHY zinc finger protein -
  ETT59_RS01115 (ETT59_01115) - 191787..192326 (+) 540 WP_002986314.1 biotin transporter BioY -
  ETT59_RS01120 (ETT59_01120) - 192466..193245 (+) 780 WP_011528209.1 MBL fold metallo-hydrolase -
  ETT59_RS01125 (ETT59_01125) tadA 193245..193760 (+) 516 WP_002992549.1 tRNA adenosine(34) deaminase TadA -
  ETT59_RS01130 (ETT59_01130) - 194374..195606 (-) 1233 WP_136276555.1 transglutaminase domain-containing protein -
  ETT59_RS01140 (ETT59_01140) speG 196006..196710 (+) 705 WP_136276557.1 streptococcal pyrogenic exotoxin SpeG -
  ETT59_RS01145 (ETT59_01145) - 197165..198514 (+) 1350 WP_014635263.1 glucose-6-phosphate isomerase -
  ETT59_RS01150 (ETT59_01150) - 198863..200371 (-) 1509 WP_023079312.1 helix-turn-helix domain-containing protein -
  ETT59_RS01155 (ETT59_01155) - 201059..201730 (+) 672 WP_011184138.1 rhomboid family intramembrane serine protease -
  ETT59_RS01160 (ETT59_01160) galU 201829..202728 (-) 900 WP_002986125.1 UTP--glucose-1-phosphate uridylyltransferase GalU -
  ETT59_RS01165 (ETT59_01165) - 202761..203777 (-) 1017 WP_002986123.1 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase -
  ETT59_RS01170 (ETT59_01170) - 204073..204522 (+) 450 WP_002986120.1 MarR family winged helix-turn-helix transcriptional regulator -
  ETT59_RS01175 (ETT59_01175) - 204515..206221 (+) 1707 WP_136266774.1 ABC transporter ATP-binding protein -
  ETT59_RS01180 (ETT59_01180) - 206224..208008 (+) 1785 WP_136276561.1 ABC transporter ATP-binding protein -
  ETT59_RS01185 (ETT59_01185) - 208126..208893 (+) 768 WP_011054158.1 epoxyqueuosine reductase QueH -
  ETT59_RS01190 (ETT59_01190) - 209003..209449 (+) 447 WP_136109470.1 dUTP diphosphatase -
  ETT59_RS01195 (ETT59_01195) radA 209530..210891 (+) 1362 WP_136276563.1 DNA repair protein RadA Machinery gene

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49438.73 Da        Isoelectric Point: 6.0962

>NTDB_id=341681 ETT59_RS01195 WP_136276563.1 209530..210891(+) (radA) [Streptococcus pyogenes strain emm124]
MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKNARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRV
LGGGVVPGSLILIGGDPGIGKSTLLLQVSTQLANKGTVFYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRIMRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKAVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=341681 ETT59_RS01195 WP_136276563.1 209530..210891(+) (radA) [Streptococcus pyogenes strain emm124]
ATGGCAAAGAAAAAAGCAACCTTTATCTGTCAGGAGTGTGGCTACCAGTCGCCAAAGTATTTAGGGCGCTGTCCAAATTG
CTCGGCCTGGTCTTCTTTTGTGGAAGAAGTAGAGGTCAAAGAAGTCAAAAATGCCCGTGTTAGTCTAGCTGGAGAAAAGT
CCAGACCGGTTAAACTAAAAGATGTGGATAATATTAGTTATCACAGAACACAGACTGACATGTCTGAATTTAATAGAGTT
CTTGGAGGGGGTGTGGTTCCTGGAAGCTTGATTTTAATTGGTGGTGATCCAGGTATTGGAAAATCCACCTTACTCTTGCA
GGTATCCACGCAGCTCGCTAATAAGGGAACGGTTTTCTATGTGTCTGGGGAAGAATCAGCAGAGCAGATTAAACTGCGGA
GTGAGCGTCTGGGTGATATTGACAATGAATTTTACCTCTATGCCGAAACCAATATGCAAGCTATTCGCACAGAAATTGAA
AATATTAAACCAGATTTTTTAATTATTGACTCCATTCAGACCATCATGAGCCCTGATATTACAGGTGTTCAAGGATCAGT
CAGTCAGGTGAGAGAAGTCACTGCAGAATTGATGCAGCTTGCAAAGACGAACAACATTGCAACCTTTATTGTGGGTCATG
TCACCAAGGAAGGAACCTTGGCTGGGCCTCGTATGTTAGAACATATGGTGGATACCGTTCTCTACTTTGAAGGGGAAAGG
CATCACACCTTTCGCATTATGCGGGCTGTGAAAAACCGCTTTGGCTCCACTAATGAGATTGGCATCTTTGAAATGCAGTC
TGGCGGCTTGGTAGAGGTCTTAAATCCTAGTCAAGTCTTTTTAGAGGAACGCTTAGATGGGGCAACAGGATCGGCTGTCG
TTGTAACGATGGAAGGAAGTCGTCCTATTTTAGCAGAGGTGCAATCTTTAGTGACACCAACCGTTTTTGGCAATGCCAGA
CGAACCACTACGGGACTTGATTTCAATCGCGTAAGCCTAATAATGGCAGTTTTAGAAAAGCGCTGTGGTTTACTCTTACA
AAATCAAGATGCCTACCTCAAGTCGGCTGGTGGTGTCAAACTAGACGAACCTGCCATTGATTTGGCAGTCGCTGTGGCTA
TCGCATCCAGTTATAAGGAAAAACCAACCAGCCCTCAAGAAGCTTTCTTAGGCGAGATCGGGTTAACAGGTGAGATTCGG
CGAGTGACCCGTATTGAGCAACGGATTAATGAAGCAGCAAAGCTTGGATTTACCAAGGTCTATGCTCCTAAAAATGCTTT
GCAAGGCATTGATATCCCTCAAGGTATTGAGGTTGTCGGAGTGACTACGGTTGGTCAGGTTTTAAAGGCAGTTTTTAGCT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(56-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

85.43

100

0.854
  radA Streptococcus pneumoniae D39

85.21

100

0.852
  radA Streptococcus pneumoniae R6

85.21

100

0.852
  radA Streptococcus pneumoniae Rx1

85.21

100

0.852
  radA Streptococcus mitis SK321

85.21

100

0.852
  radA Streptococcus pneumoniae TIGR4

85.21

100

0.852
  radA Bacillus subtilis subsp. subtilis str. 168

60.927

100

0.609

Multiple sequence alignment