Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   F6X00_RS05775 Genome accession   NZ_CP044206
Coordinates   1234043..1235767 (+) Length   574 a.a.
NCBI ID   WP_011079397.1    Uniprot ID   A0A3Q0L3L1
Organism   Vibrio vulnificus strain Vv180806     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1229043..1240767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F6X00_RS05750 (F6X00_05865) gspM 1229447..1230097 (+) 651 WP_045596391.1 type II secretion system protein GspM -
  F6X00_RS05755 (F6X00_05870) - 1230090..1230410 (+) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  F6X00_RS05760 (F6X00_05875) mshL 1230426..1232072 (+) 1647 WP_072612424.1 pilus (MSHA type) biogenesis protein MshL -
  F6X00_RS05765 (F6X00_05880) - 1232080..1232928 (+) 849 WP_039554785.1 ExeA family protein -
  F6X00_RS05770 (F6X00_05885) - 1232925..1234046 (+) 1122 WP_182290603.1 tetratricopeptide repeat protein -
  F6X00_RS05775 (F6X00_05890) pilF 1234043..1235767 (+) 1725 WP_011079397.1 GspE/PulE family protein Machinery gene
  F6X00_RS05780 (F6X00_05895) - 1235777..1236997 (+) 1221 WP_045620604.1 type II secretion system F family protein -
  F6X00_RS05785 (F6X00_05900) - 1237001..1237462 (+) 462 WP_182290605.1 MSHA biogenesis protein MshF -
  F6X00_RS05790 (F6X00_05905) - 1237541..1238119 (+) 579 WP_085761356.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  F6X00_RS05795 (F6X00_05910) - 1238153..1238644 (+) 492 WP_182290607.1 type II secretion system protein -
  F6X00_RS05800 (F6X00_05915) - 1238701..1239186 (+) 486 WP_011079402.1 type II secretion system protein -
  F6X00_RS05805 (F6X00_05920) - 1239186..1239752 (+) 567 WP_182290609.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  F6X00_RS05810 (F6X00_05925) - 1239752..1240441 (+) 690 WP_182290611.1 PilW family protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63672.03 Da        Isoelectric Point: 5.3226

>NTDB_id=341509 F6X00_RS05775 WP_011079397.1 1234043..1235767(+) (pilF) [Vibrio vulnificus strain Vv180806]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=341509 F6X00_RS05775 WP_011079397.1 1234043..1235767(+) (pilF) [Vibrio vulnificus strain Vv180806]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGACCTTCTTGTAGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCGCAAAGAAGTGGTGGACGCAAGCTTGGCGATACGCTGATTGATTTGGGTTTTTTGTCTGAGCAACAGATGC
TGGCGTTTTTGTCTCAGCAACTTGATCTGCCTTTGATTGACTTAAATCGAGCTACGGTCGATATCGATGCAGTGCAGTTG
CTACCAGAAGTGCATGCTCGTCGTTTACGTGCATTGGTGATTGGCCGTAATGGCGACACGTTGCGCATTGCGATGAGTGA
TCCAGCGGACCTTTTTGCGCAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GACAATTGGTGGAAGGCTTTGATCGCTATTATCGCCGTACCAAAGAGATCGCCAACTTTGCCGAGCAGTTACAAGCGGAA
CACCAAGTCAACGATGCATTCGATTTCAATATCGAAGATGACGACAGCGATGAAGTGACGGTCGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATCCAGGTGGGCGCATCGGATATCCATATCGAACCGGACGCCAATGTGTTGCGCTTGCGCCAAC
GTATTGATGGTGTGTTGCACGAAACCTTGCTGAATGAAGTCAACATCGCGCCAGCATTGGTACTGAGATTGAAACTCATG
GCCAACTTGGATATTTCAGAAAAGCGTCTGCCGCAAGATGGCCGTTTTAACATCCGATCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAGTCGGTGGTAATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCCGGTTTGCCGGACGATCTTTTAGTGCGTCTGCGCCGACAGTTGAAACGTCCGCACGGCATGATCCTTGTG
ACTGGCCCTACGGGCTCTGGTAAAACCACCACGCTGTATGGTGCTCTAACAGAATTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACCGACTGTCGCGTGTCAACCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACGGTGTTAAGAACATTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTAGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGATATGGGTGCTCCGGGTTATCTGGTCGCGAGTGCCGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAGACAGAAGATCATGTTGGCGAGTCACGTAAAGCGTGGTTGGCACAACGCTTCCCGAATCAAGTG
GAAGCAGGCTTTGTGAAAGGGCGCGGTTGTCAAAACTGTAACTTAACAGGCTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAACATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTTGAGTTTGCGCATGCTGCGCGTCGTTCGC
CTGGCTATAAACCTCTATTAGCGTCGGCAATGGAGCTGGCGCTACAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.699

98.432

0.371

Multiple sequence alignment