Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ETT66_RS01215 Genome accession   NZ_CP035433
Coordinates   215261..216622 (+) Length   453 a.a.
NCBI ID   WP_111693002.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain emm65     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 210261..221622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETT66_RS01200 (ETT66_01200) - 211955..213739 (+) 1785 WP_002986115.1 ABC transporter ATP-binding protein -
  ETT66_RS01205 (ETT66_01205) - 213857..214624 (+) 768 WP_136286558.1 epoxyqueuosine reductase QueH -
  ETT66_RS01210 (ETT66_01210) - 214734..215180 (+) 447 WP_002986111.1 dUTP diphosphatase -
  ETT66_RS01215 (ETT66_01215) radA 215261..216622 (+) 1362 WP_111693002.1 DNA repair protein RadA Machinery gene
  ETT66_RS01220 (ETT66_01220) - 216811..217308 (+) 498 WP_011017310.1 carbonic anhydrase -
  ETT66_RS01225 (ETT66_01225) - 217439..218149 (+) 711 WP_011017311.1 TIGR00266 family protein -
  ETT66_RS01230 (ETT66_01230) gltX 218331..219776 (+) 1446 WP_136286559.1 glutamate--tRNA ligase -
  ETT66_RS01235 (ETT66_01235) - 220171..221517 (+) 1347 WP_002991186.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49382.69 Da        Isoelectric Point: 6.0962

>NTDB_id=341309 ETT66_RS01215 WP_111693002.1 215261..216622(+) (radA) [Streptococcus pyogenes strain emm65]
MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKNARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRV
LGGGVVPGSLILIGGDPGIGKSTLLLQVSTQLANKGPVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKAVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=341309 ETT66_RS01215 WP_111693002.1 215261..216622(+) (radA) [Streptococcus pyogenes strain emm65]
ATGGCAAAGAAAAAAGCAACCTTTATCTGTCAGGAGTGTGGCTACCAGTCGCCAAAGTATTTAGGGCGCTGTCCAAATTG
CTCGGCCTGGTCTTCTTTTGTGGAAGAAGTAGAGGTCAAAGAAGTCAAAAATGCCCGTGTTAGTCTAGCTGGAGAAAAGT
CCAGACCGGTTAAACTAAAAGATGTGGATAATATTAGTTATCACAGAACACAGACTGACATGTCTGAATTTAATAGAGTT
CTTGGAGGGGGTGTGGTTCCTGGAAGCTTGATTTTAATTGGTGGTGATCCAGGTATTGGAAAATCTACCTTACTCTTGCA
GGTATCCACGCAGCTCGCTAATAAGGGACCGGTTCTCTATGTGTCTGGGGAAGAATCAGCAGAGCAGATTAAACTGCGGA
GTGAGCGTCTGGGTGATATTGACAATGAATTTTACCTCTATGCTGAAACCAATATGCAAGCTATTCGCACAGAAATTGAA
AATATTAAACCAGATTTTTTAATTATTGACTCCATTCAGACCATCATGAGCCCTGATATTACAGGTGTTCAAGGATCAGT
CAGTCAGGTGAGAGAAGTCACTGCAGAATTGATGCAGCTTGCAAAGACGAACAACATTGCAACCTTTATTGTGGGTCATG
TCACCAAGGAAGGAACCTTGGCTGGGCCTCGTATGTTAGAACACATGGTGGATACCGTTCTCTACTTTGAAGGGGAAAGG
CATCACACCTTTCGCATTCTTCGGGCTGTGAAAAACCGCTTTGGCTCCACTAACGAGATTGGCATCTTTGAAATGCAGTC
TGGCGGCTTGGTAGAGGTCTTAAATCCTAGTCAAGTCTTTTTAGAGGAACGCTTAGATGGGGCAACAGGATCGGCTGTCG
TTGTAACGATGGAAGGAAGTCGTCCTATCTTAGCAGAGGTGCAATCTTTAGTGACACCAACCGTTTTTGGCAATGCCAGA
CGAACCACTACGGGACTTGATTTCAATCGCGTAAGCCTAATAATGGCAGTTTTAGAAAAGCGCTGTGGTTTACTCTTACA
AAATCAAGATGCCTACCTCAAGTCGGCTGGTGGTGTCAAACTAGACGAACCTGCCATTGATTTGGCAGTCGCTGTGGCTA
TCGCATCCAGTTATAAGGAAAAACCAACCAGCCCTCAAGAAGCTTTCTTAGGCGAGATCGGGTTAACAGGTGAGATTCGG
CGAGTGACCCGTATTGAGCAACGGATTAATGAAGCAGCAAAGCTTGGATTTACCAAGGTCTATGCTCCTAAAAATGCCTT
GCAAGGCATTGATATCCCTCAAGGTATTGAGGTTGTCGGAGTGACTACGGTTGGTCAGGTTTTAAAGGCAGTTTTTAGCT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(56-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

85.651

100

0.857
  radA Streptococcus pneumoniae D39

85.43

100

0.854
  radA Streptococcus pneumoniae R6

85.43

100

0.854
  radA Streptococcus pneumoniae Rx1

85.43

100

0.854
  radA Streptococcus mitis SK321

85.43

100

0.854
  radA Streptococcus pneumoniae TIGR4

85.43

100

0.854
  radA Bacillus subtilis subsp. subtilis str. 168

61.369

100

0.614

Multiple sequence alignment