Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EAT1B_RS08315 Genome accession   NC_012673
Coordinates   1602157..1603533 (-) Length   458 a.a.
NCBI ID   WP_012727704.1    Uniprot ID   A0A653USY8
Organism   Exiguobacterium sp. AT1b     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1597157..1608533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAT1B_RS08290 (EAT1b_1655) cysE 1597321..1598010 (-) 690 WP_012727699.1 serine O-acetyltransferase -
  EAT1B_RS08295 (EAT1b_1656) gltX 1598269..1599726 (-) 1458 WP_193343265.1 glutamate--tRNA ligase -
  EAT1B_RS08300 (EAT1b_1657) ispF 1599772..1600245 (-) 474 WP_012727701.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  EAT1B_RS08305 (EAT1b_1658) ispD 1600242..1600922 (-) 681 WP_012727702.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EAT1B_RS08310 (EAT1b_1659) - 1600978..1602042 (-) 1065 WP_012727703.1 PIN/TRAM domain-containing protein -
  EAT1B_RS08315 (EAT1b_1660) radA 1602157..1603533 (-) 1377 WP_012727704.1 DNA repair protein RadA Machinery gene
  EAT1B_RS08320 (EAT1b_1661) - 1603590..1606025 (-) 2436 WP_012727705.1 ATP-dependent Clp protease ATP-binding subunit -
  EAT1B_RS08325 (EAT1b_1662) - 1606050..1607093 (-) 1044 WP_012727706.1 protein arginine kinase -
  EAT1B_RS08330 (EAT1b_1663) - 1607093..1607527 (-) 435 WP_012727707.1 UvrB/UvrC motif-containing protein -
  EAT1B_RS08335 (EAT1b_1664) - 1607533..1608009 (-) 477 WP_012727708.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49845.46 Da        Isoelectric Point: 6.8954

>NTDB_id=34101 EAT1B_RS08315 WP_012727704.1 1602157..1603533(-) (radA) [Exiguobacterium sp. AT1b]
MAKIKTKYVCQSCGYESAKWMGRCPGCNEWNTLVEEFVEEKKAKRGAAFVHSSSRQLKPERLANVVSQEETRVHTNSREF
DRVLGGGIVPGSLVLVGGDPGIGKSTILLQVSAHLAHSGKKVLYISGEESLKQTKLRAERLGLPTDDLFVLAETDMLMIE
RVIDEEQPGFVIIDSIQTVYMDEIQSAPGSVSQVRECTAALMKIAKTRGIAVFIVGHVTKQGSIAGPRLLEHMVDAVLYF
EGERHHTFRILRAVKNRFGSTNEIGIFEMKESGLEEVLNPSEIFLEERTAGVSGSTVVASMEGTRTVLVELQALISPTSF
GNPRRMATGIDQNRVALLMAVLEKRSGLLLQTQDAYLKAAGGVKLDEPAIDLAICISIASSFRDRPTRPTDVCIGEVGLT
GEVRRVSRIEQRVAEAAKLGFTRAIIPKNNMGGWTAPAGIEVVGVGTVDEALQLAIPF

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=34101 EAT1B_RS08315 WP_012727704.1 1602157..1603533(-) (radA) [Exiguobacterium sp. AT1b]
ATGGCCAAAATTAAAACGAAATACGTCTGCCAATCGTGCGGATATGAATCAGCCAAGTGGATGGGGCGTTGTCCGGGTTG
TAATGAATGGAATACGCTCGTCGAAGAGTTTGTAGAAGAGAAGAAAGCCAAACGTGGAGCGGCCTTTGTGCATAGTTCAT
CGCGACAATTGAAACCGGAACGCTTAGCAAACGTCGTCTCACAAGAAGAGACGCGTGTGCATACGAATAGCCGTGAATTT
GACCGCGTGCTCGGTGGAGGGATTGTCCCAGGTTCACTCGTCTTAGTCGGAGGGGACCCAGGGATTGGGAAATCGACGAT
ACTGCTACAGGTCAGCGCTCACTTAGCCCATAGTGGTAAAAAGGTGCTGTACATCTCGGGAGAGGAATCGCTCAAACAGA
CGAAACTGCGTGCGGAACGGCTTGGCTTACCGACGGATGACTTGTTCGTACTCGCTGAGACGGACATGTTGATGATCGAA
CGGGTTATTGATGAGGAGCAACCTGGATTTGTCATCATTGACTCGATTCAAACGGTATATATGGATGAGATTCAATCAGC
GCCAGGAAGCGTGTCACAAGTTCGAGAATGTACGGCCGCCCTCATGAAAATTGCGAAAACGCGAGGGATTGCCGTCTTCA
TCGTCGGTCACGTGACGAAGCAAGGGTCGATTGCCGGCCCGCGCTTACTTGAACATATGGTCGATGCGGTACTTTATTTT
GAAGGGGAGCGTCATCATACGTTCCGTATTTTACGGGCGGTAAAAAACCGTTTTGGGTCGACGAACGAGATTGGCATTTT
CGAGATGAAAGAGAGCGGACTAGAGGAAGTGCTCAATCCGTCCGAAATATTCTTGGAGGAACGCACTGCGGGCGTATCGG
GTTCGACCGTCGTTGCCTCGATGGAAGGTACGCGGACGGTACTCGTGGAACTCCAAGCACTCATCTCTCCGACATCGTTC
GGGAACCCGAGACGTATGGCCACGGGCATCGATCAAAACCGAGTCGCGCTGCTCATGGCCGTGCTCGAAAAACGTTCCGG
CCTCTTGCTTCAGACGCAGGATGCGTATTTGAAAGCAGCCGGTGGCGTCAAACTGGACGAACCGGCGATCGACCTTGCGA
TTTGTATCTCGATTGCCTCGAGTTTCCGGGATCGTCCGACCCGTCCGACGGATGTCTGCATCGGTGAGGTCGGATTGACC
GGAGAAGTGCGAAGGGTTTCCCGAATCGAGCAACGTGTCGCTGAGGCGGCAAAACTAGGTTTTACACGTGCCATCATCCC
GAAAAATAACATGGGGGGATGGACCGCTCCGGCAGGGATTGAAGTCGTCGGGGTCGGAACAGTGGACGAAGCACTCCAGT
TGGCGATTCCGTTCTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(71-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A653USY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

73.684

99.563

0.734
  radA Streptococcus mitis SK321

61.81

98.908

0.611
  radA Streptococcus mitis NCTC 12261

61.81

98.908

0.611
  radA Streptococcus pneumoniae Rx1

61.81

98.908

0.611
  radA Streptococcus pneumoniae D39

61.81

98.908

0.611
  radA Streptococcus pneumoniae R6

61.81

98.908

0.611
  radA Streptococcus pneumoniae TIGR4

61.81

98.908

0.611

Multiple sequence alignment