Detailed information NTG0340994

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: F4V41_RS14935
• Genome accession: NZ_CP044143
• Coordinates: 2814000..2814983 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli O157 strain AR-0429
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 2809000..2819983

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F4V41_RS14915	dppA	2810300..2811907 (+)	1608	WP_001222888.1	dipeptide ABC transporter substrate-binding protein DppA	-
F4V41_RS14925	dppB	2812058..2813077 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
F4V41_RS14930	dppC	2813087..2813989 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
F4V41_RS14935	amiE	2814000..2814983 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
F4V41_RS14940	dppF	2814980..2815984 (+)	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
F4V41_RS14945	yhjV	2816014..2817285 (-)	1272	WP_001301684.1	aromatic amino acid transport family protein	-
F4V41_RS14955	ldrD	2817761..2817868 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
F4V41_RS14960	bcsG	2817955..2819634 (-)	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
F4V41_RS14965	bcsF	2819631..2819822 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=340994 F4V41_RS14935 WP_001196486.1 2814000..2814983(+) (amiE) [Escherichia coli O157 strain AR-0429]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=340994 F4V41_RS14935 WP_001196486.1 2814000..2814983(+) (amiE) [Escherichia coli O157 strain AR-0429]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATTAG
CTACAGCGTAAAACAGGGCGAAGTAGTCGGGATTGTGGGTGAGTCTGGCTCCGGTAAGTCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTGATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCGGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGTCGTCAGCGGGCGATTGACT
TGTTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATTGCGATGGCCATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCATCGTTGCCTGGCGTTGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCTTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404