Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   F4V41_RS14430 Genome accession   NZ_CP044143
Coordinates   2703577..2704245 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli O157 strain AR-0429     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2698577..2709245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F4V41_RS14400 yicC 2698596..2699459 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  F4V41_RS14405 rph 2699586..2700302 (+) 717 WP_001247093.1 ribonuclease PH -
  F4V41_RS14410 pyrE 2700368..2701009 (+) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  F4V41_RS14415 slmA 2701046..2701642 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  F4V41_RS14420 dut 2701749..2702207 (-) 459 WP_000976070.1 dUTP diphosphatase -
  F4V41_RS14425 coaBC 2702185..2703405 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  F4V41_RS14430 radC 2703577..2704245 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  F4V41_RS14435 rpmB 2704462..2704698 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  F4V41_RS14440 rpmG 2704719..2704886 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  F4V41_RS14445 mutM 2704984..2705793 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  F4V41_RS14450 coaD 2705832..2706311 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  F4V41_RS14455 waaA 2706319..2707596 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  F4V41_RS14460 rfaQ 2708009..2709067 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=340993 F4V41_RS14430 WP_001297375.1 2703577..2704245(+) (radC) [Escherichia coli O157 strain AR-0429]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=340993 F4V41_RS14430 WP_001297375.1 2703577..2704245(+) (radC) [Escherichia coli O157 strain AR-0429]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACTGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment