Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   ETL60_RS12930 Genome accession   NZ_CP035414
Coordinates   2399892..2400659 (-) Length   255 a.a.
NCBI ID   WP_014477369.1    Uniprot ID   A0A0D1L7G7
Organism   Bacillus subtilis strain SRCM103637     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2394892..2405659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETL60_RS12910 (ETL60_12910) ppsR 2395067..2395879 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  ETL60_RS12915 (ETL60_12915) ccpN 2395905..2396543 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  ETL60_RS12920 (ETL60_12920) glyS 2396676..2398715 (-) 2040 WP_046160602.1 glycine--tRNA ligase subunit beta -
  ETL60_RS12925 (ETL60_12925) glyQ 2398708..2399595 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  ETL60_RS12930 (ETL60_12930) recO 2399892..2400659 (-) 768 WP_014477369.1 DNA repair protein RecO Machinery gene
  ETL60_RS12935 (ETL60_12935) yqzL 2400696..2400839 (-) 144 WP_003230053.1 YqzL family protein -
  ETL60_RS12940 (ETL60_12940) era 2400987..2401892 (-) 906 WP_087614624.1 GTPase Era -
  ETL60_RS12945 (ETL60_12945) - 2401873..2402283 (-) 411 WP_032722130.1 cytidine deaminase -
  ETL60_RS12950 (ETL60_12950) unpK 2402402..2402773 (-) 372 WP_015384119.1 diacylglycerol kinase family protein -
  ETL60_RS12955 (ETL60_12955) ybeY 2402754..2403227 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  ETL60_RS12960 (ETL60_12960) pgpH 2403228..2405363 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=340884 ETL60_RS12930 WP_014477369.1 2399892..2400659(-) (recO) [Bacillus subtilis strain SRCM103637]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=340884 ETL60_RS12930 WP_014477369.1 2399892..2400659(-) (recO) [Bacillus subtilis strain SRCM103637]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAATTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1L7G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.608

100

0.996

Multiple sequence alignment