Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ETK71_RS13010 Genome accession   NZ_CP035411
Coordinates   2517231..2517641 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain SRCM103622     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2512231..2522641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETK71_RS12980 (ETK71_12980) - 2512318..2512458 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  ETK71_RS12985 (ETK71_12985) yqeF 2512763..2513494 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  ETK71_RS12990 (ETK71_12990) cwlH 2513746..2514498 (-) 753 WP_021480067.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ETK71_RS12995 (ETK71_12995) yqeD 2514685..2515311 (+) 627 WP_041850032.1 TVP38/TMEM64 family protein -
  ETK71_RS13000 (ETK71_13000) gnd 2515331..2516224 (-) 894 WP_041850033.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ETK71_RS13005 (ETK71_13005) yqeB 2516476..2517198 (+) 723 WP_032726205.1 hypothetical protein -
  ETK71_RS13010 (ETK71_13010) nucA/comI 2517231..2517641 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  ETK71_RS13015 (ETK71_13015) - 2517837..2518184 (+) 348 Protein_2506 sigma-70 family RNA polymerase sigma factor -
  ETK71_RS13020 (ETK71_13020) spoIVCA 2518215..2519675 (-) 1461 WP_250635477.1 site-specific DNA recombinase SpoIVCA -
  ETK71_RS22140 (ETK71_13025) - 2519633..2519811 (-) 179 Protein_2508 hypothetical protein -
  ETK71_RS13030 (ETK71_13030) arsC 2520166..2520585 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  ETK71_RS13035 (ETK71_13035) acr3 2520597..2521637 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  ETK71_RS13040 (ETK71_13040) arsK 2521660..2522100 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  ETK71_RS13045 (ETK71_13045) arsR 2522161..2522478 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=340730 ETK71_RS13010 WP_009967785.1 2517231..2517641(-) (nucA/comI) [Bacillus subtilis strain SRCM103622]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=340730 ETK71_RS13010 WP_009967785.1 2517231..2517641(-) (nucA/comI) [Bacillus subtilis strain SRCM103622]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment