Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   ETK61_RS20795 Genome accession   NZ_CP035406
Coordinates   3847226..3848371 (+) Length   381 a.a.
NCBI ID   WP_017696253.1    Uniprot ID   A0A0C3JF17
Organism   Bacillus subtilis strain SRCM103612     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3842226..3853371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETK61_RS20770 (ETK61_20775) albF 3842242..3843522 (+) 1281 WP_129138005.1 pitrilysin family protein -
  ETK61_RS20775 (ETK61_20780) albG 3843519..3844220 (+) 702 WP_129138006.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  ETK61_RS20780 (ETK61_20785) ywhL 3844226..3845602 (-) 1377 WP_015250987.1 YncE family protein -
  ETK61_RS20785 (ETK61_20790) ywhK 3845641..3846996 (-) 1356 WP_114523616.1 YncE family protein -
  ETK61_RS24050 (ETK61_20795) - 3846993..3847223 (-) 231 WP_024572000.1 hypothetical protein -
  ETK61_RS20795 (ETK61_20800) rapF 3847226..3848371 (+) 1146 WP_017696253.1 tetratricopeptide repeat protein Regulator
  ETK61_RS20800 (ETK61_20805) - 3848355..3848474 (+) 120 WP_014665774.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  ETK61_RS20805 (ETK61_20810) - 3848573..3849000 (+) 428 Protein_4030 YbaK/EbsC family protein -
  ETK61_RS20810 (ETK61_20815) speB 3849065..3849937 (-) 873 WP_003227545.1 agmatinase -
  ETK61_RS20815 (ETK61_20820) speE 3849998..3850828 (-) 831 WP_003227543.1 spermidine synthase -
  ETK61_RS20820 (ETK61_20825) pbpG 3851030..3853105 (+) 2076 WP_014481227.1 transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45593.24 Da        Isoelectric Point: 5.0684

>NTDB_id=340596 ETK61_RS20795 WP_017696253.1 3847226..3848371(+) (rapF) [Bacillus subtilis strain SRCM103612]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDRREYVLAIQFFKKAESKLVFVKDKIDKAEFFFKMSEVYYYMKQTYVS
MDYARQAFEIYSEQTTYDIRMIQCQSLFGANFLDLKQYEKAIAHFQKAFTLAKTEQQPQLMGRTLYNIALCKQNQNSFES
AIEYVKKSIQVFEGSKMLPSLPQAFFLITQIYFKLGKIDNACEYHKKGKEYSAAAGDTVYLTEFEFLESLYLSGPDEERI
EKCFVFLESKMMYADIEDFALDVAKYYHQQENYKKASHYFLKVEEARQHIQGGVNLYEMEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=340596 ETK61_RS20795 WP_017696253.1 3847226..3848371(+) (rapF) [Bacillus subtilis strain SRCM103612]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTTAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGATCGCCGTGAATATGTATTAGCCATTCAATTCTTTAAAAAAGCTGAGAGCAAGCTGGTTTTTG
TCAAAGATAAAATCGATAAAGCTGAATTCTTTTTCAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTCTCC
ATGGACTATGCACGTCAAGCATTCGAAATATACAGTGAACAGACTACATATGATATCAGAATGATTCAATGCCAGTCCTT
ATTCGGAGCCAACTTTTTGGATTTAAAACAATACGAAAAAGCAATTGCCCATTTTCAAAAAGCCTTTACACTTGCAAAAA
CAGAGCAGCAGCCCCAATTAATGGGAAGAACACTTTATAACATAGCACTCTGCAAGCAAAACCAAAACAGTTTTGAATCA
GCGATAGAGTATGTAAAAAAATCTATTCAAGTATTTGAAGGATCTAAAATGCTTCCTTCATTGCCACAAGCTTTTTTCCT
AATTACACAAATTTATTTTAAATTAGGTAAGATTGATAACGCCTGTGAATATCATAAAAAAGGAAAAGAGTATTCTGCAG
CTGCGGGAGATACCGTGTATCTAACGGAGTTTGAATTTTTGGAATCTTTATACTTGTCTGGACCGGATGAGGAACGGATA
GAAAAATGTTTTGTTTTTCTTGAAAGTAAAATGATGTATGCAGATATTGAGGACTTCGCACTTGATGTTGCTAAATATTA
TCATCAACAGGAAAATTACAAAAAAGCATCTCATTACTTTTTAAAGGTTGAAGAAGCAAGACAGCACATTCAAGGGGGGG
TGAATTTATATGAAATGGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(4-65)

Tetratricopeptide repeat (TPR_8)

(263-290)

Tetratricopeptide repeat (TPR_12)

(189-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C3JF17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

80.052

100

0.801
  rapC Bacillus subtilis subsp. subtilis str. 168

57.105

99.738

0.57

Multiple sequence alignment