Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ETK61_RS14180 Genome accession   NZ_CP035406
Coordinates   2599308..2599718 (-) Length   136 a.a.
NCBI ID   WP_095843847.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103612     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2594308..2604718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETK61_RS14150 (ETK61_14155) - 2594406..2594546 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  ETK61_RS14155 (ETK61_14160) yqeF 2594852..2595583 (-) 732 WP_029317882.1 SGNH/GDSL hydrolase family protein -
  ETK61_RS14160 (ETK61_14165) cwlH 2595835..2596587 (-) 753 WP_101169548.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ETK61_RS14165 (ETK61_14170) yqeD 2596774..2597400 (+) 627 WP_129137791.1 TVP38/TMEM64 family protein -
  ETK61_RS14170 (ETK61_14175) gnd 2597419..2598312 (-) 894 WP_041333751.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ETK61_RS14175 (ETK61_14180) yqeB 2598553..2599275 (+) 723 WP_032726205.1 hypothetical protein -
  ETK61_RS14180 (ETK61_14185) nucA/comI 2599308..2599718 (-) 411 WP_095843847.1 sporulation-specific Dnase NucB Machinery gene
  ETK61_RS14185 (ETK61_14190) sigK 2599914..2600642 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  ETK61_RS14195 (ETK61_14200) - 2600856..2602103 (+) 1248 WP_031600262.1 IS256-like element ISBsu2 family transposase -
  ETK61_RS14200 (ETK61_14205) - 2602516..2603196 (-) 681 WP_015714344.1 TetR/AcrR family transcriptional regulator C-terminal domain-containing protein -
  ETK61_RS14205 (ETK61_14210) - 2603327..2604669 (+) 1343 Protein_2741 MATE family efflux transporter -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 15000.03 Da        Isoelectric Point: 5.1853

>NTDB_id=340573 ETK61_RS14180 WP_095843847.1 2599308..2599718(-) (nucA/comI) [Bacillus subtilis strain SRCM103612]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKMLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=340573 ETK61_RS14180 WP_095843847.1 2599308..2599718(-) (nucA/comI) [Bacillus subtilis strain SRCM103612]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAATGTTGTATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment