Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   ETK61_RS13930 Genome accession   NZ_CP035406
Coordinates   2555896..2556663 (-) Length   255 a.a.
NCBI ID   WP_017696274.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103612     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2550896..2561663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETK61_RS13910 (ETK61_13915) ppsR 2551071..2551883 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  ETK61_RS13915 (ETK61_13920) ccpN 2551909..2552547 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  ETK61_RS13920 (ETK61_13925) glyS 2552680..2554719 (-) 2040 WP_014480291.1 glycine--tRNA ligase subunit beta -
  ETK61_RS13925 (ETK61_13930) glyQ 2554712..2555599 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  ETK61_RS13930 (ETK61_13935) recO 2555896..2556663 (-) 768 WP_017696274.1 DNA repair protein RecO Machinery gene
  ETK61_RS13935 (ETK61_13940) yqzL 2556700..2556843 (-) 144 WP_003230053.1 YqzL family protein -
  ETK61_RS13940 (ETK61_13945) era 2556991..2557896 (-) 906 WP_003230051.1 GTPase Era -
  ETK61_RS13945 (ETK61_13950) - 2557877..2558287 (-) 411 WP_014480293.1 cytidine deaminase -
  ETK61_RS13950 (ETK61_13955) unpK 2558406..2558777 (-) 372 WP_041351685.1 diacylglycerol kinase family protein -
  ETK61_RS13955 (ETK61_13960) ybeY 2558758..2559231 (-) 474 WP_041351686.1 rRNA maturation RNase YbeY -
  ETK61_RS13960 (ETK61_13965) pgpH 2559232..2561367 (-) 2136 WP_072557183.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29346.03 Da        Isoelectric Point: 8.3080

>NTDB_id=340570 ETK61_RS13930 WP_017696274.1 2555896..2556663(-) (recO) [Bacillus subtilis strain SRCM103612]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKTNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=340570 ETK61_RS13930 WP_017696274.1 2555896..2556663(-) (recO) [Bacillus subtilis strain SRCM103612]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGAC
AAATCCTTATTTATTTGAATTCATATTAGAGTCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992

Multiple sequence alignment