Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ETK61_RS02015 Genome accession   NZ_CP035406
Coordinates   373306..373755 (-) Length   149 a.a.
NCBI ID   WP_017696127.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103612     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 368306..378755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETK61_RS01980 (ETK61_01980) yckA 368581..369261 (-) 681 WP_129137449.1 amino acid ABC transporter permease -
  ETK61_RS01985 (ETK61_01985) yckB 369271..370134 (-) 864 WP_072557073.1 transporter substrate-binding domain-containing protein -
  ETK61_RS01990 (ETK61_01990) - 370297..370487 (+) 191 Protein_344 hypothetical protein -
  ETK61_RS01995 (ETK61_01995) - 370532..370843 (+) 312 Protein_345 RDD family protein -
  ETK61_RS02000 (ETK61_02000) yckD 370925..371257 (+) 333 WP_015252849.1 YckD family protein -
  ETK61_RS02005 (ETK61_02005) bglC 371411..372844 (+) 1434 WP_017696125.1 6-phospho-beta-glucosidase -
  ETK61_RS02010 (ETK61_02010) nin/comJ 372881..373279 (-) 399 WP_017696126.1 DNA-entry nuclease inhibitor Regulator
  ETK61_RS02015 (ETK61_02015) nucA/comI 373306..373755 (-) 450 WP_017696127.1 DNA-entry nuclease Machinery gene
  ETK61_RS02020 (ETK61_02020) tlpC 373923..375644 (-) 1722 WP_026113668.1 methyl-accepting chemotaxis protein TlpC -
  ETK61_RS02025 (ETK61_02025) hxlB 375755..376312 (-) 558 WP_017696129.1 6-phospho-3-hexuloisomerase -
  ETK61_RS02030 (ETK61_02030) hxlA 376318..376950 (-) 633 WP_017696130.1 3-hexulose-6-phosphate synthase -
  ETK61_RS02035 (ETK61_02035) hxlR 377182..377544 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16412.34 Da        Isoelectric Point: 4.5282

>NTDB_id=340528 ETK61_RS02015 WP_017696127.1 373306..373755(-) (nucA/comI) [Bacillus subtilis strain SRCM103612]
MNITTDIIKTILLVIVIIAAAAAGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=340528 ETK61_RS02015 WP_017696127.1 373306..373755(-) (nucA/comI) [Bacillus subtilis strain SRCM103612]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGCCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment