Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ES969_RS13500 Genome accession   NZ_CP035402
Coordinates   2475427..2475837 (-) Length   136 a.a.
NCBI ID   WP_129110531.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103576     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2470427..2480837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES969_RS13470 (ES969_13470) - 2470516..2470656 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  ES969_RS13475 (ES969_13475) yqeF 2470962..2471693 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  ES969_RS13480 (ES969_13480) cwlH 2471945..2472697 (-) 753 WP_129134002.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ES969_RS13485 (ES969_13485) yqeD 2472884..2473508 (+) 625 Protein_2595 TVP38/TMEM64 family protein -
  ES969_RS13490 (ES969_13490) gnd 2473527..2474420 (-) 894 WP_015251670.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ES969_RS13495 (ES969_13495) yqeB 2474672..2475394 (+) 723 WP_010886572.1 hypothetical protein -
  ES969_RS13500 (ES969_13500) nucA/comI 2475427..2475837 (-) 411 WP_129110531.1 sporulation-specific Dnase NucB Machinery gene
  ES969_RS13505 (ES969_13505) sigK 2476033..2476761 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  ES969_RS13510 (ES969_13510) - 2476761..2476859 (+) 99 WP_031600702.1 hypothetical protein -
  ES969_RS21955 - 2476856..2477068 (-) 213 Protein_2601 recombinase family protein -
  ES969_RS13520 (ES969_13520) fumC 2477287..2478675 (-) 1389 WP_014480325.1 class II fumarate hydratase -
  ES969_RS13525 (ES969_13525) - 2478842..2479732 (+) 891 WP_014480326.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14939.96 Da        Isoelectric Point: 5.1853

>NTDB_id=340270 ES969_RS13500 WP_129110531.1 2475427..2475837(-) (nucA/comI) [Bacillus subtilis strain SRCM103576]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIKDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=340270 ES969_RS13500 WP_129110531.1 2475427..2475837(-) (nucA/comI) [Bacillus subtilis strain SRCM103576]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAAGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

63.478

84.559

0.537

Multiple sequence alignment