Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   ES969_RS13250 Genome accession   NZ_CP035402
Coordinates   2431992..2432759 (-) Length   255 a.a.
NCBI ID   WP_014480292.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103576     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2426992..2437759
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES969_RS13230 (ES969_13230) ppsR 2427167..2427979 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  ES969_RS13235 (ES969_13235) ccpN 2428005..2428643 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  ES969_RS13240 (ES969_13240) glyS 2428776..2430815 (-) 2040 WP_014480291.1 glycine--tRNA ligase subunit beta -
  ES969_RS13245 (ES969_13245) glyQ 2430808..2431695 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  ES969_RS13250 (ES969_13250) recO 2431992..2432759 (-) 768 WP_014480292.1 DNA repair protein RecO Machinery gene
  ES969_RS13255 (ES969_13255) yqzL 2432796..2432939 (-) 144 WP_003230053.1 YqzL family protein -
  ES969_RS13260 (ES969_13260) era 2433087..2433992 (-) 906 WP_003230051.1 GTPase Era -
  ES969_RS13265 (ES969_13265) - 2433973..2434383 (-) 411 WP_014480293.1 cytidine deaminase -
  ES969_RS13270 (ES969_13270) unpK 2434502..2434873 (-) 372 WP_080529555.1 diacylglycerol kinase family protein -
  ES969_RS13275 (ES969_13275) ybeY 2434854..2435327 (-) 474 WP_014480295.1 rRNA maturation RNase YbeY -
  ES969_RS13280 (ES969_13280) pgpH 2435328..2437463 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=340266 ES969_RS13250 WP_014480292.1 2431992..2432759(-) (recO) [Bacillus subtilis strain SRCM103576]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPLKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=340266 ES969_RS13250 WP_014480292.1 2431992..2432759(-) (recO) [Bacillus subtilis strain SRCM103576]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAGAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGCTCAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992

Multiple sequence alignment