Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ETA18_RS13035 Genome accession   NZ_CP035400
Coordinates   2515694..2516104 (-) Length   136 a.a.
NCBI ID   WP_128993173.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103835     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2510694..2521104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETA18_RS13000 (ETA18_13000) - 2510780..2510920 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  ETA18_RS13005 (ETA18_13005) yqeF 2511226..2511957 (-) 732 WP_128993169.1 SGNH/GDSL hydrolase family protein -
  ETA18_RS13015 (ETA18_13015) cwlH 2512210..2512962 (-) 753 WP_128993171.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ETA18_RS13020 (ETA18_13020) yqeD 2513149..2513775 (+) 627 WP_038429330.1 TVP38/TMEM64 family protein -
  ETA18_RS13025 (ETA18_13025) gnd 2513794..2514687 (-) 894 WP_015714298.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ETA18_RS13030 (ETA18_13030) yqeB 2514939..2515661 (+) 723 WP_128993172.1 DUF308 domain-containing protein -
  ETA18_RS13035 (ETA18_13035) nucA/comI 2515694..2516104 (-) 411 WP_128993173.1 sporulation-specific Dnase NucB Machinery gene
  ETA18_RS13040 (ETA18_13040) - 2516300..2516647 (+) 348 Protein_2506 sigma-70 family RNA polymerase sigma factor -
  ETA18_RS13045 (ETA18_13045) spoIVCA 2516678..2518138 (-) 1461 WP_250620476.1 site-specific DNA recombinase SpoIVCA -
  ETA18_RS21835 (ETA18_13050) - 2518096..2518274 (-) 179 Protein_2508 hypothetical protein -
  ETA18_RS13055 (ETA18_13055) - 2518707..2519300 (-) 594 WP_029318098.1 TetR/AcrR family transcriptional regulator -
  ETA18_RS13060 (ETA18_13060) - 2519458..2520453 (+) 996 WP_128993175.1 acryloyl-CoA reductase -
  ETA18_RS13065 (ETA18_13065) - 2520781..2521014 (+) 234 Protein_2511 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 15012.03 Da        Isoelectric Point: 5.1853

>NTDB_id=340109 ETA18_RS13035 WP_128993173.1 2515694..2516104(-) (nucA/comI) [Bacillus subtilis strain SRCM103835]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEAHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGASSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=340109 ETA18_RS13035 WP_128993173.1 2515694..2516104(-) (nucA/comI) [Bacillus subtilis strain SRCM103835]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGCACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCAGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(42-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

60.87

84.559

0.515

Multiple sequence alignment