Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ES967_RS12900 Genome accession   NZ_CP035394
Coordinates   2497827..2498237 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain SRCM103696     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2492827..2503237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES967_RS12870 (ES967_12870) - 2492914..2493054 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  ES967_RS12875 (ES967_12875) yqeF 2493360..2494091 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  ES967_RS12880 (ES967_12880) cwlH 2494343..2495095 (-) 753 WP_046381197.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ES967_RS12885 (ES967_12885) yqeD 2495282..2495908 (+) 627 WP_046381198.1 TVP38/TMEM64 family protein -
  ES967_RS12890 (ES967_12890) gnd 2495927..2496820 (-) 894 WP_038429331.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ES967_RS12895 (ES967_12895) yqeB 2497072..2497794 (+) 723 WP_010886572.1 hypothetical protein -
  ES967_RS12900 (ES967_12900) nucA/comI 2497827..2498237 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  ES967_RS12905 (ES967_12905) sigK 2498433..2499161 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  ES967_RS12910 (ES967_12910) - 2499161..2499277 (+) 117 WP_122894874.1 hypothetical protein -
  ES967_RS12915 (ES967_12915) - 2499243..2499439 (-) 197 Protein_2484 recombinase family protein -
  ES967_RS12920 (ES967_12920) - 2499397..2499561 (-) 165 WP_080265384.1 hypothetical protein -
  ES967_RS12925 (ES967_12925) - 2499994..2500587 (-) 594 WP_029318098.1 TetR/AcrR family transcriptional regulator -
  ES967_RS12930 (ES967_12930) - 2500745..2501740 (+) 996 WP_029318099.1 acryloyl-CoA reductase -
  ES967_RS12935 (ES967_12935) - 2502068..2502301 (+) 234 Protein_2488 helix-turn-helix domain-containing protein -
  ES967_RS12940 (ES967_12940) arsC 2502512..2502931 (-) 420 WP_029318100.1 arsenate reductase (thioredoxin) -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=339796 ES967_RS12900 WP_009967785.1 2497827..2498237(-) (nucA/comI) [Bacillus subtilis strain SRCM103696]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=339796 ES967_RS12900 WP_009967785.1 2497827..2498237(-) (nucA/comI) [Bacillus subtilis strain SRCM103696]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment