Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ES967_RS01970 Genome accession   NZ_CP035394
Coordinates   366175..366624 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis strain SRCM103696     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 361175..371624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES967_RS01935 (ES967_01935) yckA 361326..362006 (-) 681 WP_024572176.1 amino acid ABC transporter permease -
  ES967_RS01940 (ES967_01940) yckB 362016..362879 (-) 864 WP_029727074.1 transporter substrate-binding domain-containing protein -
  ES967_RS01945 (ES967_01945) - 363040..363237 (+) 198 WP_128992271.1 hypothetical protein -
  ES967_RS01950 (ES967_01950) yckC 363257..363712 (+) 456 WP_048654748.1 RDD family protein -
  ES967_RS01955 (ES967_01955) yckD 363794..364126 (+) 333 WP_015252849.1 YckD family protein -
  ES967_RS01960 (ES967_01960) bglC 364280..365713 (+) 1434 WP_048654749.1 6-phospho-beta-glucosidase -
  ES967_RS01965 (ES967_01965) nin/comJ 365750..366148 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  ES967_RS01970 (ES967_01970) nucA/comI 366175..366624 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  ES967_RS01975 (ES967_01975) tlpC 366792..368513 (-) 1722 WP_061891260.1 methyl-accepting chemotaxis protein TlpC -
  ES967_RS01980 (ES967_01980) hxlB 368624..369181 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  ES967_RS01985 (ES967_01985) hxlA 369187..369819 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  ES967_RS01990 (ES967_01990) hxlR 370052..370414 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=339746 ES967_RS01970 WP_003234608.1 366175..366624(-) (nucA/comI) [Bacillus subtilis strain SRCM103696]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=339746 ES967_RS01970 WP_003234608.1 366175..366624(-) (nucA/comI) [Bacillus subtilis strain SRCM103696]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATAATAGCAGCTGCAGCTGTCGGTCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment