Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   ES966_RS12585 Genome accession   NZ_CP035393
Coordinates   2550664..2551101 (-) Length   145 a.a.
NCBI ID   WP_007612572.1    Uniprot ID   -
Organism   Bacillus velezensis strain SRCM103691     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2545664..2556101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES966_RS12535 (ES966_12535) sinI 2546047..2546220 (+) 174 WP_014418369.1 anti-repressor SinI family protein Regulator
  ES966_RS12540 (ES966_12540) sinR 2546254..2546589 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  ES966_RS12545 (ES966_12545) - 2546637..2547422 (-) 786 WP_017418136.1 TasA family protein -
  ES966_RS12550 (ES966_12550) - 2547487..2548071 (-) 585 WP_012117977.1 signal peptidase I -
  ES966_RS12555 (ES966_12555) tapA 2548043..2548714 (-) 672 WP_025649852.1 amyloid fiber anchoring/assembly protein TapA -
  ES966_RS12560 (ES966_12560) - 2548973..2549302 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  ES966_RS12565 (ES966_12565) - 2549343..2549522 (-) 180 WP_003153093.1 YqzE family protein -
  ES966_RS12570 (ES966_12570) comGG 2549579..2549956 (-) 378 WP_025649851.1 competence type IV pilus minor pilin ComGG Machinery gene
  ES966_RS12575 (ES966_12575) comGF 2549957..2550352 (-) 396 WP_025649850.1 competence type IV pilus minor pilin ComGF -
  ES966_RS12580 (ES966_12580) comGE 2550366..2550680 (-) 315 WP_017418140.1 competence type IV pilus minor pilin ComGE Machinery gene
  ES966_RS12585 (ES966_12585) comGD 2550664..2551101 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  ES966_RS12590 (ES966_12590) comGC 2551091..2551399 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  ES966_RS12595 (ES966_12595) comGB 2551404..2552441 (-) 1038 WP_014418377.1 competence type IV pilus assembly protein ComGB Machinery gene
  ES966_RS12600 (ES966_12600) comGA 2552428..2553498 (-) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  ES966_RS12605 (ES966_12605) - 2553654..2554642 (-) 989 Protein_2430 magnesium transporter CorA family protein -
  ES966_RS12610 (ES966_12610) - 2554788..2556089 (+) 1302 WP_017418142.1 hemolysin family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16254.76 Da        Isoelectric Point: 10.1850

>NTDB_id=339706 ES966_RS12585 WP_007612572.1 2550664..2551101(-) (comGD) [Bacillus velezensis strain SRCM103691]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPVCTHLAVRQKTEQLQKDIQLAQETAIAEHKRTKITFLPKEHKYK
LQSGGRIVERSFDSLHITLVTLPESLEFNEKGHPNSGGKIQLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=339706 ES966_RS12585 WP_007612572.1 2550664..2551101(-) (comGD) [Bacillus velezensis strain SRCM103691]
TTGAACAATAACAGGCGGACAGAAAATGGGTTTACCCTTCTTGAAAGCCTGATTGTGTTAAGCCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGTCTGCACCCACCTTGCCGTGCGGCAAAAAACAGAACAGCTCCAAAAAGATA
TTCAGCTTGCTCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAAAGAGCATAAATACAAA
CTGCAGTCAGGCGGAAGGATTGTCGAGCGTTCTTTTGATTCGCTTCACATTACGCTTGTGACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATACAATTGAAGAGCGCCGGGTTCACCTATGAGATCACAGTTT
ACTTAGGGAGCGGAAATGTCCATGCTAAACGGAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

55.479

100

0.559

Multiple sequence alignment