Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ES965_RS12950 Genome accession   NZ_CP035391
Coordinates   2492618..2493028 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain SRCM103689     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2487618..2498028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES965_RS12920 (ES965_12920) - 2487704..2487844 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  ES965_RS12925 (ES965_12925) yqeF 2488150..2488881 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  ES965_RS12930 (ES965_12930) cwlH 2489133..2489885 (-) 753 WP_129092545.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ES965_RS12935 (ES965_12935) yqeD 2490073..2490699 (+) 627 WP_038429330.1 TVP38/TMEM64 family protein -
  ES965_RS12940 (ES965_12940) gnd 2490718..2491611 (-) 894 WP_129092546.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ES965_RS12945 (ES965_12945) yqeB 2491863..2492585 (+) 723 WP_024572277.1 hypothetical protein -
  ES965_RS12950 (ES965_12950) nucA/comI 2492618..2493028 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  ES965_RS12955 (ES965_12955) sigK 2493224..2493952 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  ES965_RS12960 (ES965_12960) - 2493952..2494067 (+) 116 Protein_2491 hypothetical protein -
  ES965_RS12965 (ES965_12965) - 2494033..2494230 (-) 198 Protein_2492 recombinase family protein -
  ES965_RS12970 (ES965_12970) - 2494188..2494352 (-) 165 WP_129092547.1 hypothetical protein -
  ES965_RS12975 (ES965_12975) yrkL 2494808..2495332 (-) 525 WP_003246192.1 NAD(P)H-dependent oxidoreductase -
  ES965_RS12980 (ES965_12980) yrkK 2495580..2496056 (-) 477 WP_004398792.1 HXXEE domain-containing protein -
  ES965_RS12985 (ES965_12985) - 2496500..2497492 (-) 993 WP_129092548.1 multidrug resistance efflux transporter family protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=339635 ES965_RS12950 WP_009967785.1 2492618..2493028(-) (nucA/comI) [Bacillus subtilis strain SRCM103689]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=339635 ES965_RS12950 WP_009967785.1 2492618..2493028(-) (nucA/comI) [Bacillus subtilis strain SRCM103689]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment