Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   ES965_RS12700 Genome accession   NZ_CP035391
Coordinates   2449176..2449943 (-) Length   255 a.a.
NCBI ID   WP_015384118.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103689     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2444176..2454943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES965_RS12680 (ES965_12680) ppsR 2444350..2445162 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  ES965_RS12685 (ES965_12685) ccpN 2445188..2445826 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  ES965_RS12690 (ES965_12690) glyS 2445959..2447998 (-) 2040 WP_129092537.1 glycine--tRNA ligase subunit beta -
  ES965_RS12695 (ES965_12695) glyQ 2447991..2448878 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  ES965_RS12700 (ES965_12700) recO 2449176..2449943 (-) 768 WP_015384118.1 DNA repair protein RecO Machinery gene
  ES965_RS12705 (ES965_12705) yqzL 2449980..2450123 (-) 144 WP_003230053.1 YqzL family protein -
  ES965_RS12710 (ES965_12710) era 2450271..2451176 (-) 906 WP_129092538.1 GTPase Era -
  ES965_RS12715 (ES965_12715) - 2451157..2451567 (-) 411 WP_003230049.1 cytidine deaminase -
  ES965_RS12720 (ES965_12720) unpK 2451686..2452057 (-) 372 WP_015384119.1 diacylglycerol kinase family protein -
  ES965_RS12725 (ES965_12725) ybeY 2452038..2452511 (-) 474 WP_024572410.1 rRNA maturation RNase YbeY -
  ES965_RS12730 (ES965_12730) pgpH 2452512..2454647 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29332.01 Da        Isoelectric Point: 8.3080

>NTDB_id=339631 ES965_RS12700 WP_015384118.1 2449176..2449943(-) (recO) [Bacillus subtilis strain SRCM103689]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKSNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=339631 ES965_RS12700 WP_015384118.1 2449176..2449943(-) (recO) [Bacillus subtilis strain SRCM103689]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGTC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTTTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTCTACTATTTTGATCTGTCGAGACTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992

Multiple sequence alignment