Detailed information NTG0339472

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ES964_RS21970
• Genome accession: NZ_CP035383
• Coordinates: 4463165..4463965 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain AP8555
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4458165..4468965

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ES964_RS21935 (ES964_21935)	gspH	4458321..4458836 (+)	516	WP_004183045.1	type II secretion system minor pseudopilin GspH	-
ES964_RS21940 (ES964_21940)	gspI	4458833..4459198 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
ES964_RS21945 (ES964_21945)	gspJ	4459195..4459791 (+)	597	WP_012737414.1	type II secretion system minor pseudopilin GspJ	-
ES964_RS21950 (ES964_21950)	gspK	4459788..4460774 (+)	987	WP_077250502.1	type II secretion system minor pseudopilin GspK	-
ES964_RS21955 (ES964_21955)	gspL	4460761..4461954 (+)	1194	WP_012737412.1	type II secretion system protein GspL	-
ES964_RS21960 (ES964_21960)	-	4461947..4462432 (+)	486	WP_012737411.1	type II secretion system protein M	-
ES964_RS21965 (ES964_21965)	-	4462422..4463168 (+)	747	WP_129075011.1	type II secretion system protein N	-
ES964_RS21970 (ES964_21970)	pilD	4463165..4463965 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
ES964_RS21975 (ES964_21975)	hrpB	4464013..4466442 (-)	2430	WP_012737409.1	ATP-dependent helicase HrpB	-
ES964_RS21980 (ES964_21980)	thpR	4466515..4467051 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
ES964_RS21985 (ES964_21985)	sfsA	4467051..4467767 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
ES964_RS21990 (ES964_21990)	dksA	4467930..4468385 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=339472 ES964_RS21970 WP_004145898.1 4463165..4463965(+) (pilD) [Klebsiella pneumoniae strain AP8555]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=339472 ES964_RS21970 WP_004145898.1 4463165..4463965(+) (pilD) [Klebsiella pneumoniae strain AP8555]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425