Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ES815_RS07220 Genome accession   NZ_CP035382
Coordinates   1311222..1312655 (+) Length   477 a.a.
NCBI ID   WP_142487257.1    Uniprot ID   -
Organism   Leclercia adecarboxylata strain R25     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1306222..1317655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES815_RS07190 (ES815_07230) - 1306789..1307766 (+) 978 WP_142487252.1 calcium/sodium antiporter -
  ES815_RS07195 (ES815_07235) kdsD 1307780..1308766 (+) 987 WP_142487253.1 arabinose-5-phosphate isomerase KdsD -
  ES815_RS07200 (ES815_07240) kdsC 1308781..1309347 (+) 567 WP_106994894.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ES815_RS07205 (ES815_07245) lptC 1309344..1309919 (+) 576 WP_142487254.1 LPS export ABC transporter periplasmic protein LptC -
  ES815_RS07210 (ES815_07250) lptA 1309888..1310442 (+) 555 WP_142487255.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ES815_RS07215 (ES815_07255) lptB 1310449..1311174 (+) 726 WP_142487256.1 LPS export ABC transporter ATP-binding protein -
  ES815_RS07220 (ES815_07260) rpoN/rpoN1 1311222..1312655 (+) 1434 WP_142487257.1 RNA polymerase factor sigma-54 Machinery gene
  ES815_RS07225 (ES815_07265) hpf 1312678..1312965 (+) 288 WP_142487258.1 ribosome hibernation promoting factor -
  ES815_RS07230 (ES815_07270) ptsN 1313048..1313539 (+) 492 WP_039031625.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ES815_RS07235 (ES815_07275) rapZ 1313585..1314439 (+) 855 WP_142487259.1 RNase adapter RapZ -
  ES815_RS07240 (ES815_07280) npr 1314436..1314708 (+) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  ES815_RS07245 (ES815_07285) mtgA 1314705..1315430 (-) 726 WP_370649949.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ES815_RS07250 (ES815_07290) elbB 1315427..1316080 (-) 654 WP_142487260.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54203.98 Da        Isoelectric Point: 4.3155

>NTDB_id=339443 ES815_RS07220 WP_142487257.1 1311222..1312655(+) (rpoN/rpoN1) [Leclercia adecarboxylata strain R25]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVETQQTQDNETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLDD
ILESIGDDEIDLEEIEAVLKRVQRFDPVGVAARDLRDCLLIQLSQFVKETPWLEEARLIVSEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMRPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=339443 ES815_RS07220 WP_142487257.1 1311222..1312655(+) (rpoN/rpoN1) [Leclercia adecarboxylata strain R25]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCTATTCGTCTGTTGCA
GCTGTCAACGCTGGAACTTCAGCAGGAACTCCAGCAGGCGCTGGACAGTAATCCCCTGCTTGAGCAAACCGATCTTCACG
ATGAGGTAGAAACTCAGCAAACCCAGGATAACGAAACTCTCGACAGCGTTGATGCGCTGGAGCAAAAAGAGATGCCTGAC
GAGCTTCCTCTCGATGCCAGCTGGGATGAGATCTACACCGCCGGCACGCCATCCGGCACGCGCGCAGACTACCAGGATGA
TGAATTGCCGGTGTATCAGGGCGAGACTACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAGCTGACGCCCTTTT
CCGATACCGATCGCGCCATCGCCACCTCTATTGTCGATGCCGTCGATGAGACCGGCTATTTGACCGTGTCGCTGGACGAC
ATTCTGGAAAGCATCGGTGATGATGAGATCGATCTCGAAGAGATCGAAGCGGTTCTGAAGCGCGTTCAGCGTTTTGATCC
GGTCGGCGTCGCCGCACGCGATTTACGTGACTGCCTGCTGATTCAGCTTTCGCAGTTTGTTAAAGAGACGCCGTGGCTGG
AAGAGGCTCGCCTGATCGTCAGCGAGCATCTGGATCTGCTGGCTAACCACGACTTCCGCTCCCTGATGCGCGTGACGCGG
CTGAAGGAAGAGATCCTGAAAGAAGCGGTCAATTTGATTCAGTCGCTGGATCCGCGTCCGGGGCAGTCTATCCAGACCAG
CGAGCCCGAGTACGTCATTCCCGATGTACTGGTGCGCAAGCATAACGGCCTGTGGACCGTGGAATTGAATTCCGACAGCA
TCCCTCGCCTGCAAATAAATCAGCAGTACGCCTCGATGTGCACCAGCGCGCGCAACGACTCGGATAACCAATATATCCGC
AGTAATCTTCAGGAAGCGCGCTGGCTAATCAAGAGCCTGGAGAGCCGCAATGATACGCTGCTGCGCGTCAGCCGTTGCAT
CGTGGAGCAGCAGCAGGCGTTCTTTGAACAAGGTGAAGAATTTATGAGGCCGATGGTGCTGGCCGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATTTCCCGCGTGACGACGCAGAAGTATCTGCATAGTCCGCGCGGTATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAACACCGAAGGCGGCGGCGAAGCTTCGTCGACGGCCATCCGGGCGCTGGTGAAAAAGTT
GATCGCCGCGGAAAACCCCGCGAAACCACTGAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAAGGTATCATGGTTG
CACGCCGCACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482

Multiple sequence alignment