Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FS841_RS18080 Genome accession   NZ_CP043937
Coordinates   3761271..3762767 (-) Length   498 a.a.
NCBI ID   WP_001040619.1    Uniprot ID   -
Organism   Escherichia coli strain RM14516     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3756271..3767767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FS841_RS18055 (FS841_18190) panM 3756492..3756875 (+) 384 WP_000778800.1 aspartate 1-decarboxylase autocleavage activator PanM -
  FS841_RS18060 (FS841_18195) livJ 3757075..3758178 (-) 1104 WP_001021986.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  FS841_RS18065 (FS841_18200) rpoH 3758450..3759304 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  FS841_RS18070 (FS841_18205) ftsX 3759549..3760607 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  FS841_RS18075 (FS841_18210) ftsE 3760600..3761268 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  FS841_RS18080 (FS841_18215) pilA 3761271..3762767 (-) 1497 WP_001040619.1 signal recognition particle-docking protein FtsY Machinery gene
  FS841_RS18085 (FS841_18220) rsmD 3762917..3763513 (+) 597 WP_000743195.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  FS841_RS18090 (FS841_18225) yhhL 3763503..3763772 (+) 270 WP_001295207.1 DUF1145 family protein -
  FS841_RS18095 (FS841_18230) yhhM 3763775..3764134 (-) 360 WP_000042883.1 DUF2500 domain-containing protein -
  FS841_RS18100 (FS841_18235) yhhN 3764275..3764901 (+) 627 WP_000964718.1 lysoplasmalogenase -
  FS841_RS18105 (FS841_18240) zntA 3764975..3767173 (+) 2199 WP_000106497.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  FS841_RS18110 (FS841_18245) - 3767206..3767514 (-) 309 WP_000385056.1 hypothetical protein -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54668.50 Da        Isoelectric Point: 4.1468

>NTDB_id=339409 FS841_RS18080 WP_001040619.1 3761271..3762767(-) (pilA) [Escherichia coli strain RM14516]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVPAQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVVIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=339409 FS841_RS18080 WP_001040619.1 3761271..3762767(-) (pilA) [Escherichia coli strain RM14516]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCCGGCGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGTTAT
CGAGCGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAACCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAGCAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTAGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAGGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTTCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378