Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   ERW10_RS10815 Genome accession   NZ_CP035369
Coordinates   2064592..2066220 (+) Length   542 a.a.
NCBI ID   WP_001186608.1    Uniprot ID   -
Organism   Staphylococcus aureus strain LAC     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 2057559..2072986 2064592..2066220 within 0


Gene organization within MGE regions


Location: 2057559..2072986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ERW10_RS10790 (ERW10_10295) - 2057559..2059049 (-) 1491 WP_000554504.1 RNA-binding domain-containing protein -
  ERW10_RS10795 (ERW10_10300) - 2059722..2060771 (-) 1050 WP_000613248.1 hypothetical protein -
  ERW10_RS10800 (ERW10_10305) ssb 2060910..2061200 (+) 291 WP_001036438.1 cassette chromosome ssDNA-binding protein -
  ERW10_RS10805 (ERW10_10310) cch1 2061200..2062987 (+) 1788 WP_000122620.1 cassette chromosome replicative helicase -
  ERW10_RS10810 (ERW10_10315) ccrA 2063221..2064570 (+) 1350 WP_000815640.1 cassette chromosome recombinase CcrA Machinery gene
  ERW10_RS10815 (ERW10_10320) ccrB 2064592..2066220 (+) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  ERW10_RS10820 (ERW10_10325) - 2066742..2067092 (+) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  ERW10_RS10830 (ERW10_10330) - 2067179..2067490 (+) 312 WP_000700851.1 DUF960 family protein -
  ERW10_RS10835 (ERW10_10335) - 2067505..2068011 (+) 507 WP_001092189.1 DUF1643 domain-containing protein -
  ERW10_RS10840 (ERW10_10340) - 2068060..2069670 (+) 1611 Protein_1999 IS1182 family transposase -
  ERW10_RS10845 (ERW10_10345) - 2069661..2069906 (-) 246 Protein_2000 type I restriction enzyme endonuclease domain-containing protein -
  ERW10_RS10850 (ERW10_10350) - 2069905..2070880 (-) 976 Protein_2001 M56 family metallopeptidase -
  ERW10_RS10855 (ERW10_10355) mecA 2070977..2072986 (+) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62654.83 Da        Isoelectric Point: 9.7970

>NTDB_id=339396 ERW10_RS10815 WP_001186608.1 2064592..2066220(+) (ccrB) [Staphylococcus aureus strain LAC]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDIAYKQQQYD
EVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKTLFDAKEISKLLQHIFHDIKHIEKSRLK
ALYLSVIDRIDIKKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=339396 ERW10_RS10815 WP_001186608.1 2064592..2066220(+) (ccrB) [Staphylococcus aureus strain LAC]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAAGTTAAAGATATATACGCTGACCGTGGTA
TTTCAGGTAAATCTATGAACAGACCTGAGCTCCAACGTATGTTGAAAGATGCAAAAGAAGGCAATATCGACTGTGTTATG
GTATACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGATTTACACAAACAAAATGTCGA
ATTTTTCAGTTTGTCAGAGCGTATGGAAGTCAATACTTCTTCTGGTAAACTCATGTTACAGATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAACGTATTTATGGGTCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
TTACCACTAGGTTATGACAAAATACCAGATAGTAAACACGAGCTAATGATTAACCAACATGAAGCTAATATTGTAAAATA
TATATTCGAGTGCTATGCCAAAGGACATGGCTATCGTAAAATTGCCAATGCATTGAATCACAAAGGATATGTCACTAAAA
AGGGGAAACCTTTCAGTATTAGTTCAATCACATACATCTTAGCTAACCCTTTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTGAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAGAAACAAGTCAGTCAAAAACCCCAAGTCCATGGCAAAGGAACGA
ATCTGCTTACAGGCATTATTCACTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACCACGAATACTCTTAAAGAC
GGGACTAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTGATGAAGCAAATACTTGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTAG
TAACACACGTCAATCAAGAAAATCAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
GAAGTACAAATCAAAATAAATAACTTGATTAAAACCATCGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGACTT
TATTTGATGCCAAAGAAATCAGTAAACTATTACAACACATCTTTCATGATATTAAGCACATAGAAAAATCTCGACTCAAA
GCATTGTATCTATCAGTGATTGATCGCATTGATATTAAAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATTAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTATTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(180-280)

Resolvase, N terminal domain (Resolvase)

(11-156)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

98.893

100

0.989

Multiple sequence alignment