Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   F1719_RS16430 Genome accession   NZ_CP043852
Coordinates   3291166..3292662 (-) Length   498 a.a.
NCBI ID   WP_001040664.1    Uniprot ID   -
Organism   Escherichia coli ATCC 8739     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3286166..3297662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F1719_RS16405 (F1719_16785) panM 3286389..3286772 (+) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
  F1719_RS16410 (F1719_16790) livJ 3286971..3288074 (-) 1104 WP_001022007.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  F1719_RS16415 (F1719_16795) rpoH 3288345..3289199 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  F1719_RS16420 (F1719_16800) ftsX 3289444..3290502 (-) 1059 WP_001042017.1 permease-like cell division protein FtsX -
  F1719_RS16425 (F1719_16805) ftsE 3290495..3291163 (-) 669 WP_000617726.1 cell division ATP-binding protein FtsE -
  F1719_RS16430 (F1719_16810) pilA 3291166..3292662 (-) 1497 WP_001040664.1 signal recognition particle-docking protein FtsY Machinery gene
  F1719_RS16435 (F1719_16815) rsmD 3292812..3293408 (+) 597 WP_000743204.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  F1719_RS16440 (F1719_16820) yhhL 3293398..3293667 (+) 270 WP_001295207.1 DUF1145 family protein -
  F1719_RS16445 (F1719_16825) yhhM 3293670..3294029 (-) 360 WP_000042886.1 DUF2500 domain-containing protein -
  F1719_RS16450 (F1719_16830) yhhN 3294170..3294796 (+) 627 WP_000964718.1 lysoplasmalogenase -
  F1719_RS16455 (F1719_16835) zntA 3294870..3297068 (+) 2199 WP_000106517.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  F1719_RS16460 (F1719_16840) tusA 3297170..3297415 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54698.53 Da        Isoelectric Point: 4.1560

>NTDB_id=339183 F1719_RS16430 WP_001040664.1 3291166..3292662(-) (pilA) [Escherichia coli ATCC 8739]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAQEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=339183 F1719_RS16430 WP_001040664.1 3291166..3292662(-) (pilA) [Escherichia coli ATCC 8739]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTAGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGCAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTAGTTCCTCCGGCAGAAGAAGAGCAGCCGGTTGAAGAAATTGCCCAGGA
GCAAGAAAAACCGACCAAAGAAGGCTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGTTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATAATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTAGGTGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACATTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGTCTGCAGAACAAATCGCATCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGTTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378

Multiple sequence alignment