Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EQ030_RS11070 Genome accession   NZ_CP035294
Coordinates   2272791..2274167 (-) Length   458 a.a.
NCBI ID   WP_011303850.1    Uniprot ID   Q49V35
Organism   Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292 strain ATCC 15305     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2267791..2279167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQ030_RS11055 (EQ030_11060) cysE 2268817..2269458 (-) 642 WP_041079568.1 serine O-acetyltransferase -
  EQ030_RS11060 (EQ030_11065) gltX 2269805..2271259 (-) 1455 WP_011303848.1 glutamate--tRNA ligase -
  EQ030_RS11065 (EQ030_11070) - 2271677..2272735 (-) 1059 WP_138936974.1 PIN/TRAM domain-containing protein -
  EQ030_RS11070 (EQ030_11075) radA 2272791..2274167 (-) 1377 WP_011303850.1 DNA repair protein RadA Machinery gene
  EQ030_RS11075 (EQ030_11080) - 2274515..2276977 (-) 2463 WP_011303851.1 ATP-dependent Clp protease ATP-binding subunit -
  EQ030_RS11080 (EQ030_11085) - 2276989..2277999 (-) 1011 WP_011303852.1 protein arginine kinase -
  EQ030_RS11085 (EQ030_11090) - 2277992..2278543 (-) 552 WP_011303853.1 UvrB/UvrC motif-containing protein -
  EQ030_RS11090 (EQ030_11095) - 2278560..2279021 (-) 462 WP_011303854.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50593.26 Da        Isoelectric Point: 8.0081

>NTDB_id=339034 EQ030_RS11070 WP_011303850.1 2272791..2274167(-) (radA) [Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292 strain ATCC 15305]
MAKKKVIFECMACGYQSPKWMGKCPNCGGWNQMEEIVEQKAASPKHGVRSRDNVSKVQKLNEIKHETTPRVKTNSNEFNR
VLGGGIVNGSLVLIGGDPGIGKSTLLLQICASLSQSKNVLYITGEESLSQTKLRAERLDEDSSELNVFAETDLEVIHETV
KQIKPDLLVVDSIQTIFHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
EHHAYRILRAVKNRFGSTNEMGIFEMKQTGLKGVKNPSEMFLEERSANVPGSTIVSTMEGTRPLLIEVQALVTPTTFNNP
RRMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVRLTEPAVDLGIIIATASSFKDLTVDGLDCFIGEVGLTGEV
RRVSRIEQRVQEAEKLGFKRVIIPESNIGGWQFPEGIKVIGVKSVHEALKYALTKHKK

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=339034 EQ030_RS11070 WP_011303850.1 2272791..2274167(-) (radA) [Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292 strain ATCC 15305]
TTGGCTAAAAAGAAAGTGATTTTTGAATGTATGGCATGTGGTTATCAGTCACCGAAATGGATGGGGAAATGCCCAAACTG
TGGTGGTTGGAATCAAATGGAAGAAATTGTCGAACAAAAGGCAGCCAGTCCAAAACATGGCGTACGCAGTCGAGACAATG
TATCTAAAGTACAAAAATTAAATGAAATTAAACATGAAACAACACCTAGGGTTAAAACAAATTCAAACGAATTTAACCGC
GTACTAGGTGGAGGTATTGTTAATGGTTCACTTGTCTTAATTGGTGGTGATCCTGGTATTGGGAAATCGACATTACTATT
ACAAATTTGTGCATCATTATCCCAATCTAAAAACGTATTATATATCACTGGTGAAGAATCATTGAGTCAAACGAAATTAA
GAGCGGAAAGATTAGATGAGGATTCTAGTGAATTAAATGTATTTGCCGAAACAGATTTAGAAGTAATCCATGAAACGGTT
AAACAAATTAAACCAGATTTACTTGTTGTGGATTCTATACAAACAATATTCCACCCTGAAATCAGCTCGGCACCAGGCTC
GGTTTCACAAGTTAGAGAAAGTACGCAGAGCTTAATGAATATAGCTAAACAGATGAATATTGCGACGTTTATTGTCGGAC
ATGTAACAAAAGAAGGTCAAATCGCTGGGCCGCGTTTACTTGAACATATGGTGGACACAGTGCTTTACTTCGAAGGTGAT
GAACACCATGCATATCGTATTTTAAGAGCTGTGAAAAACCGTTTTGGTTCTACAAATGAAATGGGTATCTTTGAGATGAA
GCAAACAGGTTTGAAAGGCGTTAAAAACCCTTCGGAAATGTTTCTTGAAGAACGTTCAGCCAATGTACCAGGTTCCACGA
TTGTTTCTACCATGGAAGGTACGAGACCTTTATTGATTGAAGTACAAGCATTAGTGACACCGACAACTTTTAATAATCCG
AGACGTATGGCAACGGGCATAGATCATAATCGATTGAGTTTGCTCATGGCCGTATTAGAAAAGAAAGAAAATTATCTCCT
TCAGCAGCAAGATGCTTATATCAAAGTTGCGGGTGGTGTGAGACTTACTGAGCCAGCAGTAGATTTAGGTATCATTATTG
CGACGGCTTCAAGCTTTAAAGATTTAACGGTGGACGGCTTGGACTGTTTCATCGGTGAGGTAGGTCTTACTGGAGAAGTC
AGACGTGTTTCTCGAATAGAACAACGTGTCCAAGAAGCTGAAAAATTAGGGTTCAAACGTGTGATTATCCCTGAGTCCAA
TATTGGGGGATGGCAATTTCCTGAAGGTATAAAAGTAATTGGGGTTAAAAGTGTTCATGAAGCTTTAAAATACGCATTAA
CAAAACATAAAAAATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(360-427)

AAA domain (AAA_25)

(67-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q49V35

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.789

99.563

0.655
  radA Streptococcus pneumoniae Rx1

60

98.253

0.59
  radA Streptococcus pneumoniae D39

60

98.253

0.59
  radA Streptococcus pneumoniae R6

60

98.253

0.59
  radA Streptococcus pneumoniae TIGR4

60

98.253

0.59
  radA Streptococcus mitis NCTC 12261

60

98.253

0.59
  radA Streptococcus mitis SK321

60

98.253

0.59

Multiple sequence alignment