Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   F1745_RS00525 Genome accession   NZ_CP043825
Coordinates   117410..117850 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain RM8082     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 112410..122850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F1745_RS00500 (F1745_00495) coaE 112900..113520 (-) 621 WP_001269527.1 dephospho-CoA kinase -
  F1745_RS00505 - 113545..113589 (+) 45 WP_120795372.1 protein YacM -
  F1745_RS00510 (F1745_00500) guaC 113745..114788 (+) 1044 WP_001217338.1 GMP reductase -
  F1745_RS00515 (F1745_00505) hofC 114823..116025 (-) 1203 WP_000157234.1 protein transport protein HofC -
  F1745_RS00520 (F1745_00510) pilB 116015..117400 (-) 1386 WP_032214586.1 type II secretion system protein GspE Machinery gene
  F1745_RS00525 (F1745_00515) pilA 117410..117850 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  F1745_RS00530 (F1745_00520) nadC 118053..118946 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  F1745_RS00535 (F1745_00525) ampD 119034..119585 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  F1745_RS00540 (F1745_00530) ampE 119582..120435 (+) 854 Protein_107 beta-lactamase regulator AmpE -
  F1745_RS00545 (F1745_00535) aroP 120478..121851 (-) 1374 WP_000969914.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=338961 F1745_RS00525 WP_000360904.1 117410..117850(-) (pilA) [Escherichia coli strain RM8082]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=338961 F1745_RS00525 WP_000360904.1 117410..117850(-) (pilA) [Escherichia coli strain RM8082]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363