Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   EQH15_RS09700 Genome accession   NZ_CP035265
Coordinates   1873809..1874825 (-) Length   338 a.a.
NCBI ID   WP_074196785.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain TVO_1901920     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1868809..1879825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQH15_RS10875 - 1868886..1869017 (-) 132 WP_000768904.1 hypothetical protein -
  EQH15_RS09660 (EQH15_10155) - 1869018..1870208 (-) 1191 WP_000167766.1 acetate kinase -
  EQH15_RS09665 (EQH15_10160) comYH 1870259..1871212 (-) 954 WP_000345122.1 class I SAM-dependent methyltransferase Machinery gene
  EQH15_RS09670 (EQH15_10165) - 1871273..1871867 (-) 595 Protein_1902 class I SAM-dependent methyltransferase -
  EQH15_RS09675 (EQH15_10170) comGG/cglG 1872004..1872417 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  EQH15_RS09680 (EQH15_10175) comGF/cglF 1872395..1872856 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  EQH15_RS09685 (EQH15_10180) comGE/cglE 1872819..1873121 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  EQH15_RS09690 (EQH15_10185) comGD/cglD 1873084..1873488 (-) 405 WP_000588026.1 competence type IV pilus minor pilin ComGD Machinery gene
  EQH15_RS09695 (EQH15_10190) comGC/cglC 1873481..1873807 (-) 327 WP_000738626.1 comG operon protein ComGC Machinery gene
  EQH15_RS09700 (EQH15_10195) comGB/cglB 1873809..1874825 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  EQH15_RS09705 (EQH15_10200) comGA/cglA/cilD 1874773..1875714 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  EQH15_RS09710 (EQH15_10205) - 1875790..1876155 (-) 366 WP_000286415.1 DUF1033 family protein -
  EQH15_RS09715 (EQH15_10210) - 1876306..1877364 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  EQH15_RS09720 (EQH15_10215) nagA 1877527..1878678 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38392.40 Da        Isoelectric Point: 9.4802

>NTDB_id=338728 EQH15_RS09700 WP_074196785.1 1873809..1874825(-) (comGB/cglB) [Streptococcus pneumoniae strain TVO_1901920]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEV
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=338728 EQH15_RS09700 WP_074196785.1 1873809..1874825(-) (comGB/cglB) [Streptococcus pneumoniae strain TVO_1901920]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAGTC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

100

100

1
  comGB/cglB Streptococcus pneumoniae D39

100

100

1
  comGB/cglB Streptococcus pneumoniae R6

100

100

1
  comGB/cglB Streptococcus pneumoniae TIGR4

100

100

1
  comGB/cglB Streptococcus mitis SK321

95.266

100

0.953
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.547

94.083

0.541
  comYB Streptococcus mutans UA159

57.547

94.083

0.541
  comGB Lactococcus lactis subsp. cremoris KW2

51.198

98.817

0.506

Multiple sequence alignment