Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   F0316_RS11865 Genome accession   NZ_CP043554
Coordinates   2544601..2546328 (-) Length   575 a.a.
NCBI ID   WP_119780857.1    Uniprot ID   -
Organism   Vibrio cholerae strain RFB16     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2539601..2551328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0316_RS11830 (F0316_11830) - 2539881..2540651 (-) 771 WP_149560501.1 PilW family protein -
  F0316_RS11835 (F0316_11835) - 2540651..2541217 (-) 567 WP_001144518.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  F0316_RS11840 (F0316_11840) - 2541207..2541719 (-) 513 WP_149560502.1 type II secretion system protein -
  F0316_RS11845 (F0316_11845) - 2541783..2542244 (-) 462 WP_000828589.1 type II secretion system protein -
  F0316_RS11850 (F0316_11850) - 2542279..2542833 (-) 555 WP_000738707.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  F0316_RS11855 (F0316_11855) - 2542914..2543366 (-) 453 WP_032468787.1 hypothetical protein -
  F0316_RS11860 (F0316_11860) - 2543369..2544592 (-) 1224 WP_000190444.1 type II secretion system F family protein -
  F0316_RS11865 (F0316_11865) pilB/pilB1 2544601..2546328 (-) 1728 WP_119780857.1 GspE/PulE family protein Machinery gene
  F0316_RS11870 (F0316_11870) - 2546318..2547469 (-) 1152 WP_162796892.1 lipopolysaccharide assembly protein LapB -
  F0316_RS11875 (F0316_11875) - 2547466..2548311 (-) 846 WP_000281360.1 ExeA family protein -
  F0316_RS11880 (F0316_11880) mshL 2548316..2549995 (-) 1680 WP_001227124.1 pilus (MSHA type) biogenesis protein MshL -
  F0316_RS11885 (F0316_11885) - 2550010..2550327 (-) 318 WP_000252812.1 MSHA biogenesis protein MshK -
  F0316_RS11890 (F0316_11890) gspM 2550320..2550970 (-) 651 WP_000835144.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63814.26 Da        Isoelectric Point: 6.0455

>NTDB_id=338407 F0316_RS11865 WP_119780857.1 2544601..2546328(-) (pilB/pilB1) [Vibrio cholerae strain RFB16]
MPINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRAHVDIDAVP
LLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNQLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHA
EHKTNDSFDFEITDADSDEVTVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKL
MANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGIPPHLLLRLRHQLKRPHGMIL
VTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISRINQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETV
EIGLRAALTGHLVLSTLHTNDAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLGTRFPNQ
VAAKFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPLLASAMELALQGIVSLDEVMS
LGEGDSSGLVEPIYL

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=338407 F0316_RS11865 WP_119780857.1 2544601..2546328(-) (pilB/pilB1) [Vibrio cholerae strain RFB16]
GTGCCAATTAATAAACTGCGTAAACGGCTTGGTGACTTGCTGGTTGAAGAGGGGATTGTCAGCGAAGCGCAGCTTGAACA
AGCGCTCAATGCCCAGAAAAATACCGGACGCCGCTTAGGGGATACCTTAATTTCGCTTGGCTTTTTAAGCGAAACCCAGT
TGCTGAACTTTTTGGCGCAGCAATTGAGTTTACCGGTGATCGATTTGAGCCGCGCTCATGTCGATATTGATGCGGTTCCT
TTACTGCCTGAAGTTCATGCCCGCCGTTTGCGTGCGCTGGTGATAGGCCGCAGTGGTGATACGCTGCGCATAGCCATGAG
TGATCCTGCGGATCTGTTTGCTCAAGAAGCCTTGCTCAACCAGTTGCCTGATTACGGTTTTGAGTTTGTCATCGCCCCTG
AGAAGCAGTTGGTCGATGGCTTTGATCGTTACTATCGCCGCACCAAAGAAATTGTCTCGTTTGCCGAGCAATTGCATGCC
GAACACAAAACCAATGATAGTTTTGATTTTGAGATCACCGATGCAGATAGCGATGAAGTGACGGTTGTTAAACTGATCAA
CTCGCTGTTTGAAGATGCGATTCAAGTGGGAGCCTCGGATATACACATTGAGCCGGATGCCAACGTACTGCGTTTGCGTC
AACGGATCGATGGGGTATTGCATGAAACCTTGCTCCATGAAGTGAATATCGCCTCAGCCTTAGTGCTGCGCTTAAAGTTA
ATGGCGAATCTGGATATTTCAGAAAAACGCCTTCCGCAAGATGGCCGCTTTAACATCCGTGTAAAAGGACAATCCGTCGA
TATCCGGATGTCAACCATGCCTATCCAGCACGGTGAATCGGTGGTGATGCGTTTGCTCAACCAATCTGCAGGGGTTAGAA
AGCTCGAAGAGTCAGGTATTCCGCCTCATTTATTGCTGCGTTTGCGCCATCAGTTGAAACGCCCTCATGGCATGATTCTG
GTGACTGGGCCTACCGGCTCGGGGAAAACCACCACCTTGTATGGCGCACTCAACGAGCTCAATACGTCCGGCAAAAAAAT
CATTACTGCGGAAGATCCGGTGGAATACCGGATTTCACGGATCAATCAGGTGCAAGTGAACCCGAAAATCAATCTCGATT
TCTCGACGTTACTGCGCACTTTCTTGCGCCAAGACCCAGATATTATTCTGGTCGGTGAGATGCGTGACCAAGAGACGGTC
GAAATTGGCCTGCGCGCCGCCTTAACCGGTCACTTAGTCTTAAGTACTCTGCACACCAATGATGCCGTCGATAGCGCGCT
GCGTTTGATTGATATGGGGGCGCCCGGTTATCTGGTTGCCAGTGCGGTACGTGCCGTGGTTGCGCAGCGTTTAGTACGAA
AAGTCTGTCCGGATTGTAGTGGACATGATGAGGTGGATGAAGCGCGTCGCCAATGGCTTGGGACGCGTTTCCCTAATCAA
GTTGCCGCCAAATTCACTCGTGGACGCGGCTGTCAAAACTGTAACTTAACTGGTTATCGCGGCCGGATCGGTGTGTTTGA
AATGCTTGAGCTGGATCAGCCGATGATGGATTGCTTGCGGGCAAATGATGCTGTGGCCTTCTCTAAAGCCGCACGCAGTA
ACACAGATTATAAACCGCTACTGGCTTCGGCGATGGAGTTAGCGCTACAAGGCATCGTCAGTCTTGATGAAGTGATGTCG
CTGGGTGAAGGTGATTCCTCTGGTTTGGTTGAACCGATTTATCTGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-149)

Type II/IV secretion system protein (T2SSE)

(189-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

35.265

100

0.37
  pilB Vibrio campbellii strain DS40M4

36.972

98.783

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.658

96.522

0.363
  pilF Thermus thermophilus HB27

37.388

97.217

0.363
  pilB Legionella pneumophila strain ERS1305867

39.771

90.957

0.362
  pilB Vibrio cholerae strain A1552

36.945

97.913

0.362
  pilB Vibrio parahaemolyticus RIMD 2210633

36.189

99.478

0.36

Multiple sequence alignment