Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CPH39_RS19955 Genome accession   NZ_CP043542
Coordinates   4122841..4123281 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain F2_81     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4117841..4128281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPH39_RS19935 (CPH39_20270) aroP 4118839..4120212 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  CPH39_RS19940 (CPH39_20275) ampE 4120255..4121109 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  CPH39_RS19945 (CPH39_20280) ampD 4121106..4121657 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CPH39_RS19950 (CPH39_20285) nadC 4121745..4122638 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CPH39_RS19955 (CPH39_20290) pilA 4122841..4123281 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  CPH39_RS19960 (CPH39_20295) pilB 4123291..4124676 (+) 1386 WP_053883415.1 type II secretion system protein GspE Machinery gene
  CPH39_RS19965 (CPH39_20300) hofC 4124666..4125868 (+) 1203 WP_089637291.1 protein transport protein HofC -
  CPH39_RS19970 (CPH39_20305) guaC 4125903..4126946 (-) 1044 WP_001217349.1 GMP reductase -
  CPH39_RS19975 - 4127102..4127146 (-) 45 WP_120795372.1 protein YacM -
  CPH39_RS19980 (CPH39_20310) coaE 4127171..4127791 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=338314 CPH39_RS19955 WP_000360904.1 4122841..4123281(+) (pilA) [Escherichia coli strain F2_81]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=338314 CPH39_RS19955 WP_000360904.1 4122841..4123281(+) (pilA) [Escherichia coli strain F2_81]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363