Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   CPH39_RS01250 Genome accession   NZ_CP043542
Coordinates   288341..289105 (-) Length   254 a.a.
NCBI ID   WP_089637315.1    Uniprot ID   -
Organism   Escherichia coli strain F2_81     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 283341..294105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPH39_RS01225 (CPH39_01245) yhhJ 285197..286321 (+) 1125 WP_001216257.1 ABC transporter permease -
  CPH39_RS01230 (CPH39_01250) - 286425..286655 (+) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  CPH39_RS01235 (CPH39_01255) - 286652..287011 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  CPH39_RS01240 (CPH39_01260) nikR 287131..287532 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  CPH39_RS01245 (CPH39_01265) nikE 287538..288344 (-) 807 WP_053883607.1 nickel import ATP-binding protein NikE -
  CPH39_RS01250 (CPH39_01270) amiE 288341..289105 (-) 765 WP_089637315.1 nickel import ATP-binding protein NikD Regulator
  CPH39_RS01255 (CPH39_01275) nikC 289105..289938 (-) 834 WP_096220430.1 nickel ABC transporter permease subunit NikC -
  CPH39_RS01260 (CPH39_01280) nikB 289935..290879 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  CPH39_RS01265 (CPH39_01285) nikA 290879..292453 (-) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  CPH39_RS01270 (CPH39_01290) acpT 292564..293151 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26843.42 Da        Isoelectric Point: 6.6882

>NTDB_id=338263 CPH39_RS01250 WP_089637315.1 288341..289105(-) (amiE) [Escherichia coli strain F2_81]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAAALGILPAGVRQTAGEILADGKAVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPTDDATLTAALEAVGLENAQRVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=338263 CPH39_RS01250 WP_089637315.1 288341..289105(-) (amiE) [Escherichia coli strain F2_81]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGGCGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAAGCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTGCATACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGCTGGGGAAACCCACCGATGACGCTACGCTTACCGCTGCCTTAGAAGCGGTGGGGCTGGAAAACGCTCAGCGTGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGATGTGGCGGTGA
TGTCGCACGGTAAAATTGTCGAACAGGGCGATGTCGAAACGCTGTTTAACGCCCCCAAACATGCGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.2

98.425

0.406
  amiE Streptococcus thermophilus LMD-9

41.2

98.425

0.406
  amiE Streptococcus salivarius strain HSISS4

40

98.425

0.394
  oppD Streptococcus mutans UA159

39.744

92.126

0.366

Multiple sequence alignment