Detailed information NTG0337353

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: EQH35_RS04475
• Genome accession: NZ_CP035245
• Coordinates: 868258..868908 (+)
• Length: 216 a.a.
• NCBI ID: WP_000387335.1
• Uniprot ID: A0A0B7MF65
• Organism: Streptococcus pneumoniae strain TVO_1901943
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 863258..873908

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQH35_RS04445 (EQH35_04685)	-	863742..863957 (+)	216	WP_001232082.1	YozE family protein	-
EQH35_RS04450 (EQH35_04690)	-	864043..865011 (+)	969	WP_000658183.1	PhoH family protein	-
EQH35_RS04455 (EQH35_04700)	-	865204..865704 (+)	501	WP_000566982.1	GNAT family N-acetyltransferase	-
EQH35_RS04460 (EQH35_04705)	-	865707..866032 (+)	326	Protein_897	TfoX/Sxy family protein	-
EQH35_RS04465	ald	866333..867444 (-)	1112	Protein_898	alanine dehydrogenase	-
EQH35_RS04470 (EQH35_04725)	-	867621..868190 (+)	570	WP_238125046.1	GNAT family N-acetyltransferase	-
EQH35_RS04475 (EQH35_04730)	comEA/celA/cilE	868258..868908 (+)	651	WP_000387335.1	helix-hairpin-helix domain-containing protein	Machinery gene
EQH35_RS04480 (EQH35_04735)	comEC/celB	868892..871132 (+)	2241	WP_000942414.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EQH35_RS04485 (EQH35_04740)	-	871311..871499 (+)	189	WP_001809102.1	hypothetical protein	-
EQH35_RS04490 (EQH35_04745)	-	871531..872118 (+)	588	WP_000933542.1	ATP-binding cassette domain-containing protein	-
EQH35_RS04495 (EQH35_04750)	-	872122..873297 (+)	1176	WP_000655957.1	ABC transporter permease	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23229.61 Da
• Isoelectric Point: 5.2344

>NTDB_id=337353 EQH35_RS04475 WP_000387335.1 868258..868908(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain TVO_1901943]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTNGEEAVSQQTGLGTASSISKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=337353 EQH35_RS04475 WP_000387335.1 868258..868908(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain TVO_1901943]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGCGGATT
TTTCCTGCTAAAACCAGCTCCACAAACACCTGTCAAAGAGACGAATTTGCAGGCTGAAGTCGCAGCTGTTTCCAAGGATT
TGGTATCCGAAAAGGAAGTGAACAAGGAAGAAAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAATGGAG
AAGAAGCAGTTAGCCAACAGACTGGTTTGGGGACAGCTTCTTCAATAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCT
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATCGACCATCGTGAGGCAAATGG
CAAGTTCAAATCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGCCAAGACTATAGAAAAGTTAAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-214)

• SLBB domain (SLBB) (76-126)

3D structure

• AlphaFold DB: A0A0B7MF65

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae Rx1	99.074	100	0.991
comEA/celA/cilE	Streptococcus pneumoniae D39	99.074	100	0.991
comEA/celA/cilE	Streptococcus pneumoniae R6	99.074	100	0.991
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	96.296	100	0.963
comEA/celA/cilE	Streptococcus mitis NCTC 12261	96.296	100	0.963
comEA/celA/cilE	Streptococcus mitis SK321	90.278	100	0.903
comEA	Lactococcus lactis subsp. cremoris KW2	43.172	100	0.454
comEA	Bacillus subtilis subsp. subtilis str. 168	41.053	87.963	0.361