Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   EQZ01_RS13355 Genome accession   NZ_CP035231
Coordinates   2550071..2550481 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain SRCM103571     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context


Location: 2545071..2555481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQZ01_RS13325 (EQZ01_13325) - 2545158..2545298 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  EQZ01_RS13330 (EQZ01_13330) yqeF 2545604..2546335 (-) 732 WP_128738026.1 SGNH/GDSL hydrolase family protein -
  EQZ01_RS13335 (EQZ01_13335) cwlH 2546587..2547339 (-) 753 WP_128738027.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  EQZ01_RS13340 (EQZ01_13340) yqeD 2547526..2548152 (+) 627 WP_128738028.1 TVP38/TMEM64 family protein -
  EQZ01_RS13345 (EQZ01_13345) gnd 2548172..2549065 (-) 894 WP_046160611.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  EQZ01_RS13350 (EQZ01_13350) yqeB 2549317..2550039 (+) 723 WP_128738029.1 DUF308 domain-containing protein -
  EQZ01_RS13355 (EQZ01_13355) nucA/comI 2550071..2550481 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  EQZ01_RS13360 (EQZ01_13360) sigK 2550677..2551405 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  EQZ01_RS13365 (EQZ01_13365) - 2551405..2551521 (+) 117 WP_041850087.1 hypothetical protein -
  EQZ01_RS13370 (EQZ01_13370) - 2551487..2551684 (-) 198 Protein_2582 recombinase family protein -
  EQZ01_RS21890 (EQZ01_13375) - 2551642..2551820 (-) 179 Protein_2583 hypothetical protein -
  EQZ01_RS13380 (EQZ01_13380) fumC 2551945..2553333 (-) 1389 WP_128738030.1 class II fumarate hydratase -
  EQZ01_RS13385 (EQZ01_13385) - 2553500..2554390 (+) 891 WP_014480326.1 LysR family transcriptional regulator -

Sequence


Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=336470 EQZ01_RS13355 WP_009967785.1 2550071..2550481(-) (nucA/comI) [Bacillus subtilis strain SRCM103571]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=336470 EQZ01_RS13355 WP_009967785.1 2550071..2550481(-) (nucA/comI) [Bacillus subtilis strain SRCM103571]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529


Multiple sequence alignment