Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EQW70_RS14155 Genome accession   NZ_CP035226
Coordinates   2671960..2672370 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain SRCM103517     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2666960..2677370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQW70_RS14125 (EQW70_14125) - 2667046..2667186 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  EQW70_RS14130 (EQW70_14130) yqeF 2667492..2668223 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  EQW70_RS14135 (EQW70_14135) cwlH 2668475..2669227 (-) 753 WP_021480067.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  EQW70_RS14140 (EQW70_14140) yqeD 2669414..2670040 (+) 627 WP_049141194.1 TVP38/TMEM64 family protein -
  EQW70_RS14145 (EQW70_14145) gnd 2670060..2670953 (-) 894 WP_041850033.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  EQW70_RS14150 (EQW70_14150) yqeB 2671205..2671927 (+) 723 WP_049141193.1 hypothetical protein -
  EQW70_RS14155 (EQW70_14155) nucA/comI 2671960..2672370 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  EQW70_RS14160 (EQW70_14160) sigK 2672566..2673294 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  EQW70_RS14165 (EQW70_14165) - 2673294..2673410 (+) 117 WP_128740585.1 hypothetical protein -
  EQW70_RS14170 (EQW70_14170) - 2673379..2673573 (-) 195 Protein_2750 recombinase family protein -
  EQW70_RS14175 (EQW70_14175) - 2673531..2673694 (-) 164 Protein_2751 hypothetical protein -
  EQW70_RS14180 (EQW70_14180) psiE 2674235..2674651 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  EQW70_RS14185 (EQW70_14185) yrkQ 2674697..2675995 (-) 1299 WP_038829697.1 two-component system sensor histidine kinase YrkQ -
  EQW70_RS14190 (EQW70_14190) yrkP 2675982..2676677 (-) 696 WP_021480140.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=336232 EQW70_RS14155 WP_009967785.1 2671960..2672370(-) (nucA/comI) [Bacillus subtilis strain SRCM103517]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=336232 EQW70_RS14155 WP_009967785.1 2671960..2672370(-) (nucA/comI) [Bacillus subtilis strain SRCM103517]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment