Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   EQW70_RS13905 Genome accession   NZ_CP035226
Coordinates   2628516..2629283 (-) Length   255 a.a.
NCBI ID   WP_049141200.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103517     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2623516..2634283
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQW70_RS13885 (EQW70_13885) ppsR 2623690..2624502 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  EQW70_RS13890 (EQW70_13890) ccpN 2624528..2625166 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  EQW70_RS13895 (EQW70_13895) glyS 2625299..2627338 (-) 2040 WP_128740578.1 glycine--tRNA ligase subunit beta -
  EQW70_RS13900 (EQW70_13900) glyQ 2627331..2628218 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  EQW70_RS13905 (EQW70_13905) recO 2628516..2629283 (-) 768 WP_049141200.1 DNA repair protein RecO Machinery gene
  EQW70_RS13910 (EQW70_13910) yqzL 2629320..2629463 (-) 144 WP_003230053.1 YqzL family protein -
  EQW70_RS13915 (EQW70_13915) era 2629611..2630516 (-) 906 WP_003230051.1 GTPase Era -
  EQW70_RS13920 (EQW70_13920) - 2630497..2630907 (-) 411 WP_003230049.1 cytidine deaminase -
  EQW70_RS13925 (EQW70_13925) unpK 2631026..2631397 (-) 372 WP_015384119.1 diacylglycerol kinase family protein -
  EQW70_RS13930 (EQW70_13930) ybeY 2631378..2631851 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  EQW70_RS13935 (EQW70_13935) pgpH 2631852..2633987 (-) 2136 WP_080121334.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29326.05 Da        Isoelectric Point: 8.3080

>NTDB_id=336228 EQW70_RS13905 WP_049141200.1 2628516..2629283(-) (recO) [Bacillus subtilis strain SRCM103517]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGAFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=336228 EQW70_RS13905 WP_049141200.1 2628516..2629283(-) (recO) [Bacillus subtilis strain SRCM103517]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTGCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGATTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992

Multiple sequence alignment