Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   EQV91_RS12230 Genome accession   NZ_CP035220
Coordinates   2367217..2369025 (-) Length   602 a.a.
NCBI ID   WP_047927873.1    Uniprot ID   -
Organism   Enterococcus faecium strain SRCM103470     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2362217..2374025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQV91_RS12205 (EQV91_12205) - 2363264..2364163 (-) 900 WP_002315659.1 RluA family pseudouridine synthase -
  EQV91_RS12210 (EQV91_12210) - 2364165..2364962 (-) 798 WP_002315658.1 NAD kinase -
  EQV91_RS12215 (EQV91_12215) - 2364937..2365644 (-) 708 WP_002309982.1 GTP pyrophosphokinase family protein -
  EQV91_RS12220 (EQV91_12220) - 2365775..2366350 (+) 576 WP_002315657.1 CYTH domain-containing protein -
  EQV91_RS12225 (EQV91_12225) - 2366468..2367145 (+) 678 WP_002328914.1 ClpXP adapter SpxH family protein -
  EQV91_RS12230 (EQV91_12230) pepF 2367217..2369025 (-) 1809 WP_047927873.1 oligoendopeptidase F Regulator
  EQV91_RS12235 (EQV91_12235) - 2369204..2370202 (-) 999 WP_002342266.1 competence protein CoiA family protein -
  EQV91_RS12240 (EQV91_12240) - 2370300..2370953 (-) 654 WP_002328912.1 adaptor protein MecA -
  EQV91_RS12245 (EQV91_12245) spxA 2371283..2371681 (-) 399 WP_002296384.1 transcriptional regulator SpxA -
  EQV91_RS12250 (EQV91_12250) - 2372446..2373153 (+) 708 WP_002309968.1 HAD family hydrolase -

Sequence

Protein


Download         Length: 602 a.a.        Molecular weight: 69635.04 Da        Isoelectric Point: 4.7311

>NTDB_id=336113 EQV91_RS12230 WP_047927873.1 2367217..2369025(-) (pepF) [Enterococcus faecium strain SRCM103470]
MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEKLKKSEKYKGTLDQGASQFLDAIEFVLSVYRQTEIIYVYAH
LKNDQDTGNTEYQALYARASSLFSKVSEAVSWFEPEILQLSDERIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSADQESL
LAGAGEIFEASSDTFAVLNNADLVFPTIEGENGEKVQLSHGVYGQLLESTDRSVREAAFKGLYSVYEQFRNTFASTLSTH
IKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVAVVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTY
EEAKEKALEALKPMGEEYMTIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHEMGHSVHSY
FTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKAPRVRAYVLNHYLDGFKGTVFRQTQFAEFEHFMHTEDEKGVP
LTSEYLSDSYGKLNAKYYGPAVEEDPEIKFEWSRIPHFYYNYYVFQYSTGFSAASALAKRILSQEPDALENYLAYLKAGN
SDYPVEVMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL

Nucleotide


Download         Length: 1809 bp        

>NTDB_id=336113 EQV91_RS12230 WP_047927873.1 2367217..2369025(-) (pepF) [Enterococcus faecium strain SRCM103470]
ATGGAAGTAAAGCAGTTGCCAAAACGCGAAGAATTGCCTGAAAATTTAACTTGGGACTTGACCAAGATCTTTTCAAGCGA
CCAAGAATTTGATGAGAAATATCTAGAATTATCAGAAAAGTTAAAAAAATCTGAAAAATACAAAGGAACACTTGATCAAG
GGGCTTCTCAATTTCTTGATGCGATTGAATTTGTGTTGAGTGTTTATCGCCAAACTGAAATCATTTATGTCTATGCGCAC
CTTAAAAACGATCAAGATACTGGGAATACAGAATATCAAGCGCTTTATGCAAGAGCAAGTAGTTTGTTTTCAAAGGTTAG
TGAAGCTGTTTCCTGGTTTGAACCAGAAATATTGCAGTTATCAGATGAACGGATTTGGCAATATTTCAAAGAAGAACCGA
AGTTAGAAGTCTATCGTCACTATATCCAGCAAATAGTAGATAATCGTGCCCATGTTTTATCTGCCGATCAGGAATCGCTT
CTTGCAGGAGCAGGTGAAATCTTTGAGGCTTCAAGTGATACATTTGCTGTTTTGAATAATGCAGATTTAGTTTTCCCAAC
GATCGAAGGAGAGAATGGTGAAAAAGTCCAATTATCTCATGGCGTGTACGGTCAGTTGCTAGAAAGCACGGATCGAAGTG
TTCGCGAAGCCGCATTTAAAGGGTTGTACAGTGTTTATGAACAATTTAGAAATACGTTTGCTTCTACTTTAAGTACGCAT
ATAAAAGGACATAATTTTAAAGCAAAAGTTCGTAATTACAGCTCTGCCAGAGAAGCATCTTTGAGCAATAATCATATTCC
TGAAAGTGTATACGACACTTTGGTAGCCGTGGTAAACAAGCATTTGCCTTTGCTGCATCGATATATGGAATTACGGAAAC
GTTTATTAGAAGTGGAAAAACTGCACATGTACGATCTTTATACACCGGTCTTAGGAGAAGCTCCAATCACCTTTACGTAT
GAAGAAGCAAAAGAGAAAGCTTTGGAAGCACTGAAGCCAATGGGTGAAGAGTACATGACTATTGTAGAAAAAGCATTCTC
TGAACGTTGGATCGATGTTGTCGAAAATAAAGGAAAACGAAGTGGTGCCTATTCTTCGGGAAGCTATGACACAAATCCAT
ATATTTTATTGAATTGGCATGATACATTGGATCAGCTGTTTACGCTTGTCCACGAAATGGGACATAGTGTCCACAGTTAT
TTCACACGGTCGAACCAGCCTTATGTATACGGTGACTACTCTATCTTTTTAGCAGAAATTGCTTCGACAACGAACGAAAA
TATCCTAACAGAATATTTATTGGAAACAGAAAAAGCCCCTCGTGTCCGGGCTTATGTGCTCAACCATTACTTGGATGGAT
TTAAAGGAACAGTTTTCCGCCAGACACAATTTGCTGAATTCGAGCACTTCATGCATACGGAAGATGAAAAAGGTGTGCCA
TTGACTAGTGAATATCTAAGTGATAGTTATGGTAAATTGAATGCAAAATATTATGGTCCGGCAGTCGAAGAAGATCCAGA
AATCAAATTTGAATGGTCACGGATCCCGCATTTTTACTATAATTACTATGTCTTCCAATACTCGACTGGTTTTTCTGCTG
CTTCAGCGTTGGCGAAAAGAATACTCAGTCAGGAACCAGATGCGCTAGAGAACTATTTGGCTTATCTAAAAGCTGGTAAC
AGTGATTATCCTGTTGAAGTGATGAAAAAAGCAGGAGTAGATATGACACAGGCTGCGTATATCGAAGATGCAATGTCGAT
GTTTGAACAACGACTAAACGAATTAGAAGAATTGATTGATCGTTTATAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(206-586)

Oligopeptidase F (Peptidase_M3_N)

(116-185)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

55.481

98.505

0.547

Multiple sequence alignment