Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FYK41_RS18635 Genome accession   NZ_CP043279
Coordinates   3911602..3912042 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain WCHEC025157     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3906602..3917042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYK41_RS18615 (FYK41_18770) aroP 3907600..3908973 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  FYK41_RS18620 (FYK41_18775) ampE 3909016..3909870 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  FYK41_RS18625 (FYK41_18780) ampD 3909867..3910418 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FYK41_RS18630 (FYK41_18785) nadC 3910506..3911399 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FYK41_RS18635 (FYK41_18790) pilA 3911602..3912042 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  FYK41_RS18640 (FYK41_18795) pilB 3912052..3913437 (+) 1386 WP_001025146.1 type II secretion system protein GspE Machinery gene
  FYK41_RS18645 (FYK41_18800) hofC 3913427..3914629 (+) 1203 WP_000157266.1 protein transport protein HofC -
  FYK41_RS18650 (FYK41_18805) guaC 3914664..3915707 (-) 1044 WP_001217338.1 GMP reductase -
  FYK41_RS18655 - 3915863..3915907 (-) 45 WP_120795372.1 protein YacM -
  FYK41_RS18660 (FYK41_18810) coaE 3915932..3916552 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=336065 FYK41_RS18635 WP_000360904.1 3911602..3912042(+) (pilA) [Escherichia coli strain WCHEC025157]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=336065 FYK41_RS18635 WP_000360904.1 3911602..3912042(+) (pilA) [Escherichia coli strain WCHEC025157]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363