Detailed information NTG0033599

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: BCA_RS26850
• Genome accession: NC_012472
• Coordinates: 4939745..4941094 (-)
• Length: 449 a.a.
• NCBI ID: WP_000225366.1
• Uniprot ID: A0A158RMK4
• Organism: Bacillus cereus 03BB102
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4934745..4946094

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BCA_RS26830 (BCA_5318)	secA	4935067..4937574 (-)	2508	WP_000579383.1	preprotein translocase subunit SecA	-
BCA_RS26835 (BCA_5319)	hpf	4937851..4938393 (-)	543	WP_000671189.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
BCA_RS26840 (BCA_5321)	cspC	4938717..4938914 (-)	198	WP_001990088.1	cold shock protein CspC	-
BCA_RS26845 (BCA_5322)	comFC	4939041..4939745 (-)	705	WP_003162242.1	comF operon protein ComFC	-
BCA_RS26850 (BCA_5323)	comFA	4939745..4941094 (-)	1350	WP_000225366.1	ATP-dependent helicase ComFA	Machinery gene
BCA_RS26855 (BCA_5324)	-	4941222..4942532 (-)	1311	WP_000734330.1	NlpC/P60 family protein	-
BCA_RS26860 (BCA_5325)	-	4942786..4943100 (-)	315	WP_000400857.1	winged helix-turn-helix transcriptional regulator	-
BCA_RS26870 (BCA_5326)	-	4943274..4944116 (-)	843	WP_000844766.1	DegV family protein	-
BCA_RS26875 (BCA_5327)	-	4944356..4944991 (+)	636	WP_000926685.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51428.10 Da
• Isoelectric Point: 9.6542

>NTDB_id=33599 BCA_RS26850 WP_000225366.1 4939745..4941094(-) (comFA) [Bacillus cereus 03BB102]
MVAGKQLLLEELSSDLRRELSDLKKKGEIICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGTLSTGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKEERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KERAARIYLTATPDEKWKRNFRRGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEMSLLLKGLDHRIDGVHAEDPMRKEKVEAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=33599 BCA_RS26850 WP_000225366.1 4939745..4941094(-) (comFA) [Bacillus cereus 03BB102]
ATGGTTGCTGGAAAGCAGTTACTATTAGAAGAACTCTCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGAAGGG
AGAGATCATATGTGTACAAGGCGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGAATTCATGAAAGAAAAGGAGAAAGAGAATTACATTCGTTACAGTGGAAAGGAACTTT
GTCTACTGGTCAGGAGTTGGCAGCGCAAGGTGTTATAGAAGCTATTAAGCAGAAAGAATCGTTTTTTATTTGGGCGGTAT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGAAGAAAGAGTTTGTATTGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACTCACCAACTGTTACGTTACTATAGAGCATTCC
ATGTGATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAACAAGCGATG
AAAGAGAGAGCGGCGCGTATTTATTTAACTGCAACCCCGGATGAAAAGTGGAAGCGCAATTTCAGAAGGGGGAAACAAAA
AGGTATCATTGTCTCTGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGTAATTGGAAGAAAA
GCCTTCATCATAAAAAAATTCCTCGAGTGTTACTACAATGGTTGAAAATGTACGTAAACAAAAAATATCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATGAGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGGAAGCGTTTAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGGGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCACTACGGCAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (107-254)

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

3D structure

• AlphaFold DB: A0A158RMK4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.914	93.096	0.483
comFA	Latilactobacillus sakei subsp. sakei 23K	40.5	89.087	0.361