Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FYK53_RS18320 Genome accession   NZ_CP043274
Coordinates   3764114..3764554 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain WCHEC035148     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3759114..3769554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYK53_RS18300 (FYK53_18355) aroP 3760112..3761485 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  FYK53_RS18305 (FYK53_18360) ampE 3761528..3762382 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  FYK53_RS18310 (FYK53_18365) ampD 3762379..3762930 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FYK53_RS18315 (FYK53_18370) nadC 3763018..3763911 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FYK53_RS18320 (FYK53_18375) pilA 3764114..3764554 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  FYK53_RS18325 (FYK53_18380) pilB 3764564..3765949 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  FYK53_RS18330 (FYK53_18385) hofC 3765939..3767141 (+) 1203 WP_000157227.1 protein transport protein HofC -
  FYK53_RS18335 (FYK53_18390) guaC 3767176..3768219 (-) 1044 WP_001217342.1 GMP reductase -
  FYK53_RS18340 - 3768375..3768419 (-) 45 WP_120795372.1 protein YacM -
  FYK53_RS18345 (FYK53_18395) coaE 3768444..3769064 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=335981 FYK53_RS18320 WP_000360895.1 3764114..3764554(+) (pilA) [Escherichia coli strain WCHEC035148]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=335981 FYK53_RS18320 WP_000360895.1 3764114..3764554(+) (pilA) [Escherichia coli strain WCHEC035148]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363