Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   EQJ84_RS12540 Genome accession   NZ_CP035191
Coordinates   2446794..2447561 (-) Length   255 a.a.
NCBI ID   WP_014477369.1    Uniprot ID   A0A0D1L7G7
Organism   Bacillus subtilis strain SRCM104011     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2441794..2452561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQJ84_RS12520 (EQJ84_12520) ppsR 2441968..2442780 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  EQJ84_RS12525 (EQJ84_12525) ccpN 2442806..2443444 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  EQJ84_RS12530 (EQJ84_12530) glyS 2443577..2445616 (-) 2040 WP_128441734.1 glycine--tRNA ligase subunit beta -
  EQJ84_RS12535 (EQJ84_12535) glyQ 2445609..2446496 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  EQJ84_RS12540 (EQJ84_12540) recO 2446794..2447561 (-) 768 WP_014477369.1 DNA repair protein RecO Machinery gene
  EQJ84_RS12545 (EQJ84_12545) yqzL 2447598..2447741 (-) 144 WP_003230053.1 YqzL family protein -
  EQJ84_RS12550 (EQJ84_12550) era 2447889..2448794 (-) 906 Protein_2417 GTPase Era -
  EQJ84_RS12555 (EQJ84_12555) - 2448775..2449185 (-) 411 WP_003230049.1 cytidine deaminase -
  EQJ84_RS12560 (EQJ84_12560) unpK 2449304..2449675 (-) 372 WP_046160604.1 diacylglycerol kinase family protein -
  EQJ84_RS12565 (EQJ84_12565) ybeY 2449656..2450129 (-) 474 WP_032722131.1 rRNA maturation RNase YbeY -
  EQJ84_RS12570 (EQJ84_12570) pgpH 2450130..2452265 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=335907 EQJ84_RS12540 WP_014477369.1 2446794..2447561(-) (recO) [Bacillus subtilis strain SRCM104011]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=335907 EQJ84_RS12540 WP_014477369.1 2446794..2447561(-) (recO) [Bacillus subtilis strain SRCM104011]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGTCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGTATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1L7G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.608

100

0.996

Multiple sequence alignment