Detailed information NTG0335803

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: EQJ69_RS06315
• Genome accession: NZ_CP035188
• Coordinates: 1231211..1234903 (+)
• Length: 1230 a.a.
• NCBI ID: WP_009328898.1
• Uniprot ID: -
• Organism: Bacillus licheniformis strain SRCM103914
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1226211..1239903

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQJ69_RS06305 (EQJ69_06305)	modB	1226958..1227614 (+)	657	WP_009328900.1	molybdate ABC transporter permease subunit	-
EQJ69_RS06310 (EQJ69_06310)	addB	1227724..1231224 (+)	3501	WP_009328899.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
EQJ69_RS06315 (EQJ69_06315)	addA	1231211..1234903 (+)	3693	WP_009328898.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
EQJ69_RS06320 (EQJ69_06320)	sbcD	1234968..1236149 (+)	1182	WP_009328897.1	exonuclease subunit SbcD	-
EQJ69_RS06325 (EQJ69_06325)	sbcC	1236146..1239538 (+)	3393	WP_128472348.1	exonuclease subunit SbcC	-
EQJ69_RS06330 (EQJ69_06330)	-	1239583..1239801 (-)	219	WP_016885923.1	spore germination protein	-

Sequence

Protein

• Length: 1230 a.a.
• Molecular weight: 141056.93 Da
• Isoelectric Point: 5.5198

>NTDB_id=335803 EQJ69_RS06315 WP_009328898.1 1231211..1234903(+) (addA) [Bacillus licheniformis strain SRCM103914]
MEISKPKGSTWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITDQERPVDVDRLLVVTFTNASAAEMKHRIGEA
LEKELAENPGSLHLRRQLALLNKASISTLHSFCLQVIRKYYYLIDVDPAFRIADQTEGELLGDEVLDELFEEEYKKGNPA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPEGWLHSLAELYDAASDTKVEALPFYSYIKEDIALVLEGMRQKLTRA
LDLTKQPGGPAPRAENFLDDLAQIDRLIQQQDDFSALHELVPTVSFQRLKPCKGDEYDPRLVDEAADLRNSAKKQLEKLK
SDYFSRTPEQHLESLREMKPVIQTLVQLVLEYGRRFAEAKKEKAIVDFSDLEHDCLAILSVKNPAGEAVPSEAAKFYRHQ
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDDGSGSGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVEYDEQAELKLGASYPPNEATKTELLLIETPSGAPGEEAEELEAVQLEARAMAGQIRRL
ITEKFQVYDAKAKASRNIQYRDIVVLLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEATEVSVTLSLLKIIDNAYQDIPL
ASVLRSPVVGLDENELSLIRIKDKKAPFYEAMKAYLAAADGDERLSEKLRRFDGLLKKWRAYAKNHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYEATSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLIAMKKKLRRELLSEELRVLYVALTRAKEKLYLVG
TAKDKEKLLADWRTQAAGSEWLLPDYERFQAKSYLDFIGPALMRHRDMDESGAPPVIDEIREHPARFQVSWLSAADLQAE
AVDQAGEERHDRLVQIQMGHAIEGAFEYEQQVRERLAWSYPYKDAAKVRTKQSVSEMKRQKEYEDEYGDRSLIRPSQEAL
LFKRPSFMMAKGLTAAERGTAMHTVMQHIPLTRTPEKNELSRLLDRLVEKELLTEDQRAAIEEDDILAFFDTEIGQKLLG
ARRVEREVPFNMTLSAKEVYPDLESADEPVLIQGIIDCLFETEDGFYLLDYKTDRIHGKYRNGFEGAEPILRKRYETQIQ
LYARAVETMIKMPLKGRALYFFDGGHVLLF

Nucleotide

• Length: 3693 bp

>NTDB_id=335803 EQJ69_RS06315 WP_009328898.1 1231211..1234903(+) (addA) [Bacillus licheniformis strain SRCM103914]
ATGGAGATTTCTAAACCGAAGGGCAGCACCTGGACTGATGACCAATGGAAGGCGATTGTCTCATCCGGCCGGGACATTTT
AGTTGCGGCAGCGGCCGGTTCCGGAAAAACGGCCGTGCTGGTTGAACGGATCATTCGGAAAATAACGGATCAGGAACGAC
CGGTAGATGTCGACAGGCTGCTCGTCGTCACCTTTACAAATGCATCTGCGGCTGAAATGAAGCACAGGATCGGCGAAGCG
CTCGAAAAAGAGCTTGCCGAAAATCCGGGATCGCTTCATTTGCGCCGACAGCTTGCTTTATTAAACAAAGCGAGCATATC
AACATTGCATTCATTCTGTCTTCAAGTCATCCGCAAATATTACTATTTGATCGATGTCGATCCGGCTTTCCGGATCGCCG
ATCAGACGGAAGGCGAGCTGCTCGGCGATGAGGTGCTGGATGAACTGTTTGAAGAAGAATACAAAAAAGGCAATCCTGCC
TTTTTTGAACTTGTAGACAGATATACGACAGACCGTCATGACCTCGACCTGCAGCATCTTGTCAAGCGGGTCTACGAATT
TTCCCGTTCGCATCCCGATCCGGAGGGCTGGCTTCACTCATTGGCTGAACTGTATGACGCGGCTTCGGATACGAAGGTTG
AGGCTCTGCCGTTTTATTCTTACATTAAAGAAGACATCGCACTCGTTCTGGAAGGTATGCGGCAAAAGCTGACGCGCGCG
CTTGATTTGACGAAGCAGCCCGGGGGGCCTGCTCCGCGCGCCGAAAATTTTCTCGATGATTTAGCGCAGATCGACAGGCT
GATTCAGCAGCAGGATGATTTTTCGGCCCTGCATGAGCTTGTACCGACCGTTTCGTTTCAACGATTGAAGCCATGCAAAG
GTGATGAGTACGATCCCAGATTGGTTGATGAGGCGGCAGACCTCCGCAACAGTGCGAAAAAGCAGCTTGAAAAGCTGAAA
AGCGATTATTTTTCGCGAACTCCGGAGCAGCACCTTGAGAGCCTGAGGGAAATGAAGCCTGTCATTCAGACGCTTGTCCA
GCTCGTGCTGGAGTATGGCAGGCGGTTTGCGGAAGCGAAAAAGGAAAAGGCGATCGTCGATTTTTCAGACCTTGAACACG
ACTGCCTTGCGATTTTATCCGTGAAAAATCCGGCGGGCGAGGCCGTGCCGAGCGAAGCGGCGAAGTTTTACCGCCATCAG
TTCCACGAAGTGCTTGTTGATGAATACCAGGATACGAACCTGGTCCAGGAAGCGATTTTAAAGCTCGTCGCCAAAGAGGA
GCATGAGGGGAACCTGTTTATGGTGGGCGATGTCAAGCAGTCGATTTACCGGTTCCGCCTGGCTGAGCCGCTTTTGTTCC
TCTCGAAATATAAACGCTTTACAGACGATGGCTCGGGTTCCGGCCAGAAAATCGATTTAAATAAAAACTTCAGAAGCCGT
TCAGATATTTTGGACAGCACGAACTTTTTATTCAAACAGCTCATGGGAGAAAAAGTCGGGGAAGTCGAGTATGACGAACA
AGCCGAATTGAAGCTCGGCGCCTCTTATCCGCCGAACGAAGCGACAAAAACGGAGCTTCTGCTCATCGAAACCCCGAGCG
GCGCGCCGGGTGAAGAGGCTGAAGAGCTTGAGGCGGTCCAGCTTGAAGCAAGAGCGATGGCCGGGCAGATCCGCAGGCTG
ATTACAGAAAAATTTCAAGTATATGATGCCAAAGCGAAAGCGTCGCGAAACATCCAGTACCGTGATATCGTGGTGCTTTT
GCGGTCGATGCCTTGGGCGCCTCAAATTATGGACGAGTTTAAACAGCAGGGAATACCCGTATACGCGAACCTCTCAACTG
GTTATTTTGAGGCGACAGAAGTATCGGTCACACTGTCTTTATTAAAAATCATCGATAATGCCTATCAGGACATCCCGCTT
GCGTCCGTTCTCAGATCGCCTGTTGTGGGTCTTGATGAGAATGAGCTTTCCTTGATCAGAATCAAAGATAAAAAAGCGCC
GTTTTACGAAGCCATGAAAGCGTACCTTGCTGCAGCAGACGGCGATGAGAGGCTCAGCGAAAAGCTTCGCCGCTTTGACG
GTCTGCTCAAGAAGTGGAGAGCTTATGCGAAAAACCACTCTGTAGCAGAGCTGATCTGGGAAATCTACCGGGATACGAAG
TATTTGGATTATGTCGGCGGAATGCCGGGCGGAAAACAGCGGCAGGCCAATTTGCGCGCGCTTTACGACAGGGCGAGATC
CTATGAAGCGACATCATTCCGCGGCTTGTTCCGCTTTTTAAGATTTATTGAGCGGATGCAGGAGCGGGGCGATGACCTCG
GAACGGCAAGGGCTCTAAGCGAACAGGAAGACGTTGTTCGGCTGATGACGATCCACTCAAGCAAAGGGCTTGAATTTCCT
GTCGTATTTACCGCCGGTCTCGGACGGAATTTTAATATGATGGACTTGAACAAATCTTATCTGCTGGATAAAGAGCTCGG
TTTTGGAACGAAGTTTATCCATCCGAAGTGGCGGATCAGCTATCCGACGCTGCCGCTTATCGCGATGAAGAAAAAACTCC
GCCGCGAGCTTTTATCAGAGGAGCTGCGCGTCTTGTATGTCGCCCTTACCCGGGCCAAGGAAAAGCTGTACCTGGTCGGG
ACGGCGAAGGATAAAGAAAAGCTGCTTGCCGACTGGCGGACACAAGCGGCCGGGTCCGAGTGGCTGCTCCCGGATTATGA
ACGGTTTCAGGCAAAATCATATCTGGATTTCATCGGACCTGCTTTGATGAGGCACAGAGATATGGATGAATCCGGGGCGC
CGCCTGTGATTGATGAGATCCGCGAACATCCGGCGCGCTTTCAGGTCAGCTGGCTGTCCGCCGCGGATCTACAAGCTGAG
GCTGTCGATCAGGCCGGTGAAGAAAGACACGACCGGCTCGTCCAAATTCAAATGGGCCATGCGATTGAAGGCGCCTTTGA
ATATGAGCAGCAAGTCAGAGAACGACTGGCATGGTCATATCCATACAAGGACGCCGCCAAAGTCAGAACAAAACAATCTG
TCTCAGAAATGAAACGGCAGAAGGAATACGAGGATGAATACGGCGACCGCTCGCTTATTCGTCCATCACAGGAGGCTCTC
CTGTTTAAACGGCCGTCCTTTATGATGGCAAAAGGCCTGACCGCGGCCGAACGGGGGACGGCGATGCACACCGTCATGCA
GCATATTCCGCTGACCCGTACGCCTGAAAAGAATGAGCTCAGCCGCCTTCTAGACAGGCTTGTTGAGAAAGAGCTGTTAA
CAGAAGACCAGCGGGCAGCGATTGAAGAAGACGACATTCTTGCATTCTTTGATACGGAAATCGGACAAAAGCTTCTCGGC
GCCCGCCGGGTCGAACGGGAGGTTCCCTTTAACATGACGCTGTCCGCTAAAGAGGTTTATCCGGATCTTGAGTCAGCCGA
TGAACCTGTTTTGATACAGGGAATTATCGACTGCCTATTCGAGACGGAGGACGGTTTTTACCTTCTAGATTACAAAACCG
ACCGGATACACGGAAAGTACCGGAACGGCTTCGAAGGGGCCGAGCCGATTTTAAGAAAACGTTACGAAACACAAATTCAG
CTTTACGCACGAGCCGTTGAAACGATGATTAAAATGCCGCTCAAAGGGCGCGCGCTGTACTTTTTTGACGGAGGACACGT
GCTCTTATTTTAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1051-1222)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

• UvrD-like helicase C-terminal domain (UvrD_C) (471-880)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	71.707	100	0.717