Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EQI56_RS13610 Genome accession   NZ_CP035165
Coordinates   2603032..2603442 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain SRCM103881     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2598032..2608442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQI56_RS13580 (EQI56_13580) - 2598119..2598259 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  EQI56_RS13585 (EQI56_13585) yqeF 2598564..2599295 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  EQI56_RS13590 (EQI56_13590) cwlH 2599547..2600299 (-) 753 WP_021480067.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  EQI56_RS13595 (EQI56_13595) yqeD 2600486..2601112 (+) 627 WP_041850032.1 TVP38/TMEM64 family protein -
  EQI56_RS13600 (EQI56_13600) gnd 2601132..2602025 (-) 894 WP_041850033.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  EQI56_RS13605 (EQI56_13605) yqeB 2602277..2602999 (+) 723 WP_032726205.1 hypothetical protein -
  EQI56_RS13610 (EQI56_13610) nucA/comI 2603032..2603442 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  EQI56_RS13615 (EQI56_13615) sigK 2603638..2604366 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  EQI56_RS13620 (EQI56_13620) - 2604366..2604482 (+) 117 WP_041850087.1 hypothetical protein -
  EQI56_RS13625 (EQI56_13625) - 2604448..2604645 (-) 198 Protein_2632 recombinase family protein -
  EQI56_RS13630 (EQI56_13630) - 2604603..2604767 (-) 165 WP_080332536.1 hypothetical protein -
  EQI56_RS13635 (EQI56_13635) psiE 2605312..2605728 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  EQI56_RS13640 (EQI56_13640) yrkQ 2605774..2607072 (-) 1299 WP_041850088.1 two-component system sensor histidine kinase YrkQ -
  EQI56_RS13645 (EQI56_13645) yrkP 2607059..2607754 (-) 696 WP_041850089.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=335291 EQI56_RS13610 WP_009967785.1 2603032..2603442(-) (nucA/comI) [Bacillus subtilis strain SRCM103881]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=335291 EQI56_RS13610 WP_009967785.1 2603032..2603442(-) (nucA/comI) [Bacillus subtilis strain SRCM103881]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment