Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   EQI48_RS18660 Genome accession   NZ_CP035164
Coordinates   3558379..3559770 (-) Length   463 a.a.
NCBI ID   WP_029725993.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM104005     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3553379..3564770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQI48_RS18625 (EQI48_18625) flgL 3553550..3554446 (-) 897 WP_015714797.1 flagellar hook-associated protein FlgL -
  EQI48_RS18630 (EQI48_18630) flgK 3554457..3555980 (-) 1524 WP_003228001.1 flagellar hook-associated protein FlgK -
  EQI48_RS18635 (EQI48_18635) flgN 3555999..3556481 (-) 483 WP_014481048.1 flagellar protein FlgN -
  EQI48_RS18640 (EQI48_18640) flgM 3556497..3556763 (-) 267 WP_014478128.1 flagellar biosynthesis anti-sigma factor FlgM -
  EQI48_RS18645 (EQI48_18645) yvyF 3556844..3557263 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  EQI48_RS18650 (EQI48_18650) comFC 3557337..3558059 (-) 723 WP_029725992.1 comF operon protein ComFC Machinery gene
  EQI48_RS18655 (EQI48_18655) comFB 3558023..3558319 (-) 297 WP_015483764.1 late competence protein ComFB -
  EQI48_RS18660 (EQI48_18660) comFA 3558379..3559770 (-) 1392 WP_029725993.1 ATP-dependent helicase ComFA Machinery gene
  EQI48_RS18665 (EQI48_18665) fakBA 3559876..3560721 (-) 846 WP_003227986.1 DegV family protein -
  EQI48_RS18670 (EQI48_18670) degU 3560819..3561508 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  EQI48_RS18675 (EQI48_18675) degS 3561591..3562748 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  EQI48_RS18680 (EQI48_18680) yvyE 3562965..3563618 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52575.62 Da        Isoelectric Point: 9.9619

>NTDB_id=335226 EQI48_RS18660 WP_029725993.1 3558379..3559770(-) (comFA) [Bacillus subtilis strain SRCM104005]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENEPNWQSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNKLAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=335226 EQI48_RS18660 WP_029725993.1 3558379..3559770(-) (comFA) [Bacillus subtilis strain SRCM104005]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCCTTATATTCATGGGAAGAGGAAAATGA
ACCAAACTGGCAGTCAATTAAACTGACATGGGACGGCAAGCTTTCAAGCGGACAACAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCGCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCATTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAAAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCGTCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTACTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATA
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

99.136

100

0.991

Multiple sequence alignment