Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EQI48_RS02000 Genome accession   NZ_CP035164
Coordinates   370416..370865 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis strain SRCM104005     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 365416..375865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQI48_RS01965 (EQI48_01965) yckA 365569..366249 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  EQI48_RS01970 (EQI48_01970) yckB 366259..367122 (-) 864 WP_029727074.1 transporter substrate-binding domain-containing protein -
  EQI48_RS01975 (EQI48_01975) - 367284..367481 (+) 198 WP_119122841.1 hypothetical protein -
  EQI48_RS01980 (EQI48_01980) yckC 367500..367955 (+) 456 WP_029727075.1 RDD family protein -
  EQI48_RS01985 (EQI48_01985) yckD 368037..368369 (+) 333 WP_015252849.1 YckD family protein -
  EQI48_RS01990 (EQI48_01990) bglC 368521..369954 (+) 1434 WP_029727076.1 6-phospho-beta-glucosidase -
  EQI48_RS01995 (EQI48_01995) nin/comJ 369991..370389 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  EQI48_RS02000 (EQI48_02000) nucA/comI 370416..370865 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  EQI48_RS02005 (EQI48_02005) tlpC 371033..372754 (-) 1722 WP_029727077.1 methyl-accepting chemotaxis protein TlpC -
  EQI48_RS02010 (EQI48_02010) hxlB 372865..373422 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  EQI48_RS02015 (EQI48_02015) hxlA 373428..374060 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  EQI48_RS02020 (EQI48_02020) hxlR 374292..374654 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=335165 EQI48_RS02000 WP_003234608.1 370416..370865(-) (nucA/comI) [Bacillus subtilis strain SRCM104005]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=335165 EQI48_RS02000 WP_003234608.1 370416..370865(-) (nucA/comI) [Bacillus subtilis strain SRCM104005]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment